Differences in the Bacterial Community of Laboratory and Wild Populations of the Predatory Mite Cheyletus eruditus (Acarina: Cheyletidae) and Bacteria Transmission From Its Prey Acarus siro (Acari: Acaridae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F16%3A00003658" target="_blank" >RIV/00027006:_____/16:00003658 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1093/jee/tow032" target="_blank" >http://dx.doi.org/10.1093/jee/tow032</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jee/tow032" target="_blank" >10.1093/jee/tow032</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Differences in the Bacterial Community of Laboratory and Wild Populations of the Predatory Mite Cheyletus eruditus (Acarina: Cheyletidae) and Bacteria Transmission From Its Prey Acarus siro (Acari: Acaridae)

Popis výsledku v původním jazyce

The parthenogenetic predatory mite Cheyletus eruditus (Schrank, 1781) is used for biological control against mite pests produced as CHEYLETIN. Although there is evidence that bacteria are mainly responsible for parthenogeny in several species of predatory mites, the description of association between C. eruditus the specific and parasitic or symbiotic bacteria is still missing. We analyzed the bacterial communities of the predator, C. eruditus, and its prey, Acarus siro L. The 16S rRNA gene was amplified, cloned, and sequenced. The selected bacterial taxa were confirmed by amplification of isolated DNA with taxon-specific primers. The 16S rRNA gene sequences from the predatory and prey mites formed a total of 20 different bacterial taxa. Of these taxa, the predator and prey shared four taxa, six taxa were specific for the predatory, and 10 taxa for the prey mites. Cardinium- and Bartonella-like bacteria were found in both mite species. The reproductive parasite Wolbachia was found only in the predatory mite, and A. siro hosted Solitalea-like (Sphingobacteriales) bacteria that were not detected in C. eruditus. We focused on Cardinium occurrence in the field samples of C. eruditus. Using Cardinium-specific primers, 128 clones were obtained. Cardinium was found in seven field samples of C. eruditus as well as in the laboratory population that was used to produce CHEYLETIN. Phylogenetic analysis of the Cardinium clones identified three separate clusters: two clusters showed high similarity to the Cardinium sequences from astigmatid mites, and one cluster contained only the clones from C. eruditus. Sequences of both Cardinium and Wolbachia were found in the both adults and eggs of C. eruditus, indicating maternal transfer of these endosymbiotic bacteria.

Název v anglickém jazyce

Differences in the Bacterial Community of Laboratory and Wild Populations of the Predatory Mite Cheyletus eruditus (Acarina: Cheyletidae) and Bacteria Transmission From Its Prey Acarus siro (Acari: Acaridae)

Popis výsledku anglicky

The parthenogenetic predatory mite Cheyletus eruditus (Schrank, 1781) is used for biological control against mite pests produced as CHEYLETIN. Although there is evidence that bacteria are mainly responsible for parthenogeny in several species of predatory mites, the description of association between C. eruditus the specific and parasitic or symbiotic bacteria is still missing. We analyzed the bacterial communities of the predator, C. eruditus, and its prey, Acarus siro L. The 16S rRNA gene was amplified, cloned, and sequenced. The selected bacterial taxa were confirmed by amplification of isolated DNA with taxon-specific primers. The 16S rRNA gene sequences from the predatory and prey mites formed a total of 20 different bacterial taxa. Of these taxa, the predator and prey shared four taxa, six taxa were specific for the predatory, and 10 taxa for the prey mites. Cardinium- and Bartonella-like bacteria were found in both mite species. The reproductive parasite Wolbachia was found only in the predatory mite, and A. siro hosted Solitalea-like (Sphingobacteriales) bacteria that were not detected in C. eruditus. We focused on Cardinium occurrence in the field samples of C. eruditus. Using Cardinium-specific primers, 128 clones were obtained. Cardinium was found in seven field samples of C. eruditus as well as in the laboratory population that was used to produce CHEYLETIN. Phylogenetic analysis of the Cardinium clones identified three separate clusters: two clusters showed high similarity to the Cardinium sequences from astigmatid mites, and one cluster contained only the clones from C. eruditus. Sequences of both Cardinium and Wolbachia were found in the both adults and eggs of C. eruditus, indicating maternal transfer of these endosymbiotic bacteria.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Economic Entomology

ISSN

0022-0493

e-ISSN

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Svazek periodika

109

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

1450-1457

Kód UT WoS článku

000378041400060

EID výsledku v databázi Scopus

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