New effective cryopreservation approach for grey poplar in vitro cultures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F17%3A00004388" target="_blank" >RIV/00027006:_____/17:00004388 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

New effective cryopreservation approach for grey poplar in vitro cultures

Popis výsledku v původním jazyce

Populus x canescence (grey poplar) belongs among fast-growing woody plant species. For its unique phenotype traits observed in floodplain forest in the South Moravia region of the Czech Republic and a relatively rapid growth in in vitro as well, approximately 4-6 weeks of micropropagated plants were used to test the most effective culture conditions for a long-term conservation under ultra-low temperature. Firstly, excised apical and nodal segments were followed in their growth to determine suitable plant material for cryopreservation. Apical segments exhibited more steady developmental progress opposite to unbalanced growth of nodal segments therefore modified vitrification protocol (80% PVS3 cryoprotectant) was applied into the apical segments of 2 mm length.

Název v anglickém jazyce

New effective cryopreservation approach for grey poplar in vitro cultures

Popis výsledku anglicky

Populus x canescence (grey poplar) belongs among fast-growing woody plant species. For its unique phenotype traits observed in floodplain forest in the South Moravia region of the Czech Republic and a relatively rapid growth in in vitro as well, approximately 4-6 weeks of micropropagated plants were used to test the most effective culture conditions for a long-term conservation under ultra-low temperature. Firstly, excised apical and nodal segments were followed in their growth to determine suitable plant material for cryopreservation. Apical segments exhibited more steady developmental progress opposite to unbalanced growth of nodal segments therefore modified vitrification protocol (80% PVS3 cryoprotectant) was applied into the apical segments of 2 mm length.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

<a href="/cs/project/QJ1630301" target="_blank" >QJ1630301: Tvorba nových systémů biotechnologických opatření pro zachování a rozvoj biodiverzity zemědělských plodin a lesních dřevin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů