Alliinase and cysteine synthase transcription in developing garlic (Allium sativum L.) over time

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F18%3A00004821" target="_blank" >RIV/00027006:_____/18:00004821 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.foodchem.2017.12.090" target="_blank" >http://dx.doi.org/10.1016/j.foodchem.2017.12.090</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.foodchem.2017.12.090" target="_blank" >10.1016/j.foodchem.2017.12.090</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Alliinase and cysteine synthase transcription in developing garlic (Allium sativum L.) over time

Popis výsledku v původním jazyce

Garlic is a valuable source of health-promoting compounds, including secondary metabolites rich in sulfur such as cysteine sulfoxides (CSO). In this study, a new qRT-PCR assay is presented, analyzing the transcription of two genes encoding key enzymes of CSO biosynthetic pathway (cysteine synthase and alliinase) in developing garlic. In addition, a set of three reference genes (RGs) is presented which are suitable for normalization of the transcription of the described genes of interest. Using newly introduced qRT-PCR assays unique to cysteine synthase (chloroplast and cytosol specific), alliinase, and using RGs, we have shown that the corresponding genes have been actively transcribed throughout the whole life cycle with a peak in the germination phase. Since the content of sulfur-rich metabolites in garlic bulbs is based, among other factors, on the transcription of genes involved in the sulfur metabolism pathways in the leaves, this test can be used to examine the impact of agricultural practices or the environment on the content of sulfur metabolites in harvested garlic.

Název v anglickém jazyce

Alliinase and cysteine synthase transcription in developing garlic (Allium sativum L.) over time

Popis výsledku anglicky

Garlic is a valuable source of health-promoting compounds, including secondary metabolites rich in sulfur such as cysteine sulfoxides (CSO). In this study, a new qRT-PCR assay is presented, analyzing the transcription of two genes encoding key enzymes of CSO biosynthetic pathway (cysteine synthase and alliinase) in developing garlic. In addition, a set of three reference genes (RGs) is presented which are suitable for normalization of the transcription of the described genes of interest. Using newly introduced qRT-PCR assays unique to cysteine synthase (chloroplast and cytosol specific), alliinase, and using RGs, we have shown that the corresponding genes have been actively transcribed throughout the whole life cycle with a peak in the germination phase. Since the content of sulfur-rich metabolites in garlic bulbs is based, among other factors, on the transcription of genes involved in the sulfur metabolism pathways in the leaves, this test can be used to examine the impact of agricultural practices or the environment on the content of sulfur metabolites in harvested garlic.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QJ1210158" target="_blank" >QJ1210158: Bezpečná a kvalitní zelenina r. Allium se zaměřením na česnek z domácích zdrojů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Chemistry

ISSN

0308-8146

e-ISSN

1873-7072

Svazek periodika

251

Číslo periodika v rámci svazku

JUN 15 2018

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

103-109

Kód UT WoS článku

000424511400014

EID výsledku v databázi Scopus

2-s2.0-85041470551