TaqMan Multiplex Real-Time qPCR assays for the detection and quantification of Barley yellow dwarf virus, Wheat dwarf virus and Wheat streak mosaic virus and the study of their interactions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F18%3A00005030" target="_blank" >RIV/00027006:_____/18:00005030 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1071/CP18189" target="_blank" >http://dx.doi.org/10.1071/CP18189</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1071/CP18189" target="_blank" >10.1071/CP18189</a>

Jazyk výsledku

angličtina

Název v původním jazyce

TaqMan Multiplex Real-Time qPCR assays for the detection and quantification of Barley yellow dwarf virus, Wheat dwarf virus and Wheat streak mosaic virus and the study of their interactions

Popis výsledku v původním jazyce

In the Czech Republic, Barley yellow dwarf virus (BYDV) and Wheat dwarf virus (WDV) are the prevailing viral species that can cause significant yield losses in cereal stands. Another virus, which has been on the increase in the Czech Republic and whose impact is expected to increase, is Wheat streak mosaic virus (WSMV). In this study, a TaqMan Multiplex Real-Time (qPCR) assay for simultaneous detection of BYDV (species PAV, PAS and MAV), WDV and WSMV was developed. Furthermore, additional assays for the viruses' quantification in wheat and barley were developed. The assay was optimised and used to identify possible synergistic or antagonistic relations between the viruses in wheat and barley which would be characterised by increases or decreases of the titre of the individual viruses. For this, a greenhouse experiment with artificial inoculations of mixed and single infections of BYDV-PAV, WDV and WSMV was carried out, and field samples from the wheat and barley stands with mixed and single infections of BYDV and WDV were collected. No trend of increasing or decreasing titre was recorded for any of the viruses apart from WSMV titre which was decreased approximately five times in mixed infections with BYDV or WDV compared with the single WSMV infection. Further analyses are needed to expand the idea of possible interactions between WSMV and WDV or BYDV.

Název v anglickém jazyce

TaqMan Multiplex Real-Time qPCR assays for the detection and quantification of Barley yellow dwarf virus, Wheat dwarf virus and Wheat streak mosaic virus and the study of their interactions

Popis výsledku anglicky

In the Czech Republic, Barley yellow dwarf virus (BYDV) and Wheat dwarf virus (WDV) are the prevailing viral species that can cause significant yield losses in cereal stands. Another virus, which has been on the increase in the Czech Republic and whose impact is expected to increase, is Wheat streak mosaic virus (WSMV). In this study, a TaqMan Multiplex Real-Time (qPCR) assay for simultaneous detection of BYDV (species PAV, PAS and MAV), WDV and WSMV was developed. Furthermore, additional assays for the viruses' quantification in wheat and barley were developed. The assay was optimised and used to identify possible synergistic or antagonistic relations between the viruses in wheat and barley which would be characterised by increases or decreases of the titre of the individual viruses. For this, a greenhouse experiment with artificial inoculations of mixed and single infections of BYDV-PAV, WDV and WSMV was carried out, and field samples from the wheat and barley stands with mixed and single infections of BYDV and WDV were collected. No trend of increasing or decreasing titre was recorded for any of the viruses apart from WSMV titre which was decreased approximately five times in mixed infections with BYDV or WDV compared with the single WSMV infection. Further analyses are needed to expand the idea of possible interactions between WSMV and WDV or BYDV.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/TF02000056" target="_blank" >TF02000056: NGS pro širokospektrální diagnostiku virových chorob rostlin a pro studium interakcí virus-hostitel</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Crop & Pasture Science

ISSN

1836-0947

e-ISSN

1836-5795

Svazek periodika

69

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

AU - Austrálie

Počet stran výsledku

10

Strana od-do

755-764

Kód UT WoS článku

000441026700001

EID výsledku v databázi Scopus

2-s2.0-85052631914