Molecular diversity of barley yellow dwarf virus-PAV from China and the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10149542" target="_blank" >RIV/00027006:_____/20:10149542 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2095311920632415/pdf?md5=c5843514442c01d67549d71dd89bf3ea&pid=1-s2.0-S2095311920632415-main.pdf" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2095311920632415/pdf?md5=c5843514442c01d67549d71dd89bf3ea&pid=1-s2.0-S2095311920632415-main.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/S2095-3119(20)63241-5" target="_blank" >10.1016/S2095-3119(20)63241-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular diversity of barley yellow dwarf virus-PAV from China and the Czech Republic

Popis výsledku v původním jazyce

Wheat yellow dwarf disease (BYD), caused by different species of barley/cereal yellow dwarf viruses (B/CYDVs), is one of the most serious cereal diseases in China and the Czech Republic. Because genetic diversity of the virus directly influences disease epidemiology, the molecular diversity and population structure of 24 Chinese isolates and 16 the Czech Republic isolates of BYDV-PAV from different regions in two countries were analyzed by sequencing their coat protein (CP) and readthrough protein (RTP) domain (RTD) genes and comparing the sequences with six CP and 16 RTP sequences of BYDV-PAV isolates from the NCBI database based on nucleotide identity position, phylogenetic analysis and nucleotide diversity. Nucleotide identities between the Chinese and the Czech Republic isolates for the CP were 76.6-99.4%, 73.9-89.1% for RTD (ORF5), respectively. The Chinese and the other country isolates showed 74.7-99.2% nucleotide identity for RTP (ORF3+ORF5). Phylogenetic analysis of CP sequences showed that 20 Chinese isolates clustered in the same Glade, but the other four Chinese isolates clustered in another clade with the isolates from the Czech Republic and other counties. The population of BYDV-PAV in China had greater nucleotide variability and was more divergent than that in the Czech Republic. Geographical and ecological factors but not hosts might contribute to the population differences in the two countries.

Název v anglickém jazyce

Molecular diversity of barley yellow dwarf virus-PAV from China and the Czech Republic

Popis výsledku anglicky

Wheat yellow dwarf disease (BYD), caused by different species of barley/cereal yellow dwarf viruses (B/CYDVs), is one of the most serious cereal diseases in China and the Czech Republic. Because genetic diversity of the virus directly influences disease epidemiology, the molecular diversity and population structure of 24 Chinese isolates and 16 the Czech Republic isolates of BYDV-PAV from different regions in two countries were analyzed by sequencing their coat protein (CP) and readthrough protein (RTP) domain (RTD) genes and comparing the sequences with six CP and 16 RTP sequences of BYDV-PAV isolates from the NCBI database based on nucleotide identity position, phylogenetic analysis and nucleotide diversity. Nucleotide identities between the Chinese and the Czech Republic isolates for the CP were 76.6-99.4%, 73.9-89.1% for RTD (ORF5), respectively. The Chinese and the other country isolates showed 74.7-99.2% nucleotide identity for RTP (ORF3+ORF5). Phylogenetic analysis of CP sequences showed that 20 Chinese isolates clustered in the same Glade, but the other four Chinese isolates clustered in another clade with the isolates from the Czech Republic and other counties. The population of BYDV-PAV in China had greater nucleotide variability and was more divergent than that in the Czech Republic. Geographical and ecological factors but not hosts might contribute to the population differences in the two countries.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/LTACH17010" target="_blank" >LTACH17010: Nové strategie integrované ochrany obilnin vůči virovým chorobám v České republice a Číně</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Science Direct

ISSN

2095-3119

e-ISSN

—

Svazek periodika

19

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

2736-2745

Kód UT WoS článku

000579857800013

EID výsledku v databázi Scopus

2-s2.0-85092502132