Microbiome variation during culture growth of the European house dust mite, Dermatophagoides pteronyssinus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F21%3A10149595" target="_blank" >RIV/00027006:_____/21:10149595 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/21:88582 RIV/00216224:14310/21:00122404

Výsledek na webu

<a href="https://academic.oup.com/femsec/issue/97/4" target="_blank" >https://academic.oup.com/femsec/issue/97/4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/femsec/fiab039" target="_blank" >10.1093/femsec/fiab039</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Microbiome variation during culture growth of the European house dust mite, Dermatophagoides pteronyssinus

Popis výsledku v původním jazyce

In culture, the house dust mite, Dermatophagoides pteronyssinus, shows different growth patterns, but the composition and changes in the associated microbial community during mite culture growth are poorly known. In this study, we analyzed temporal changes in microbial communities including &apos;internal&apos; communities (inside mites, ingested) and &apos;environmental&apos; communities (from culture environment). Microbial community structure was correlated with guanine content (a nitrogenous waste product of mites) and mite population density. Both internal and environmental microbial communities were remarkably consistent between biological replicates from the same culture age group and were composed of relatively few dominant taxa-11 bacterial and 3 fungal operational taxonomic units (OTUs). Significant changes over time in microbial community structure in the bulk culture environment and in internal mite samples were observed. The yeast, Saccharomyces cerevisiae, a main component of the mite diet, gradually disappeared during mite culture growth and was replaced by fungi from the genera Aspergillus and Candida in both &apos;internal&apos; and &apos;environmental&apos; samples. In environmental samples, bacteria from the genus Lactobacillus and S. cerevisiae were negatively correlated, and Aspergillus and Candida positively correlated, with guanine content. The relative abundance of bacteria from the genus Kocuria increased with mite density but declined with increasing guanine content. The relative abundance of bacteria from the genus Virgibacillus was negatively correlated with mite density in &apos;internal&apos; samples. Gram-positive bacteria dominated bacterial microbiomes at all time points in our experiments, indicating a more limited possibility for vaccine contamination by bacterial endotoxins (heat-stable lipopolysaccharides produced mostly by Gram-negative bacteria) in our experimental cultures.

Název v anglickém jazyce

Microbiome variation during culture growth of the European house dust mite, Dermatophagoides pteronyssinus

Popis výsledku anglicky

In culture, the house dust mite, Dermatophagoides pteronyssinus, shows different growth patterns, but the composition and changes in the associated microbial community during mite culture growth are poorly known. In this study, we analyzed temporal changes in microbial communities including &apos;internal&apos; communities (inside mites, ingested) and &apos;environmental&apos; communities (from culture environment). Microbial community structure was correlated with guanine content (a nitrogenous waste product of mites) and mite population density. Both internal and environmental microbial communities were remarkably consistent between biological replicates from the same culture age group and were composed of relatively few dominant taxa-11 bacterial and 3 fungal operational taxonomic units (OTUs). Significant changes over time in microbial community structure in the bulk culture environment and in internal mite samples were observed. The yeast, Saccharomyces cerevisiae, a main component of the mite diet, gradually disappeared during mite culture growth and was replaced by fungi from the genera Aspergillus and Candida in both &apos;internal&apos; and &apos;environmental&apos; samples. In environmental samples, bacteria from the genus Lactobacillus and S. cerevisiae were negatively correlated, and Aspergillus and Candida positively correlated, with guanine content. The relative abundance of bacteria from the genus Kocuria increased with mite density but declined with increasing guanine content. The relative abundance of bacteria from the genus Virgibacillus was negatively correlated with mite density in &apos;internal&apos; samples. Gram-positive bacteria dominated bacterial microbiomes at all time points in our experiments, indicating a more limited possibility for vaccine contamination by bacterial endotoxins (heat-stable lipopolysaccharides produced mostly by Gram-negative bacteria) in our experimental cultures.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/GA17-12068S" target="_blank" >GA17-12068S: Regulují asociované bakterie v interakci s metabolity populace prachových roztočů?</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FEMS MICROBIOLOGY ECOLOGY

ISSN

0168-6496

e-ISSN

—

Svazek periodika

97

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

16

Strana od-do

fiab039

Kód UT WoS článku

000644508800016

EID výsledku v databázi Scopus

2-s2.0-85103683715