Guidance document on multiplex real-time PCR methods

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F21%3A10174364" target="_blank" >RIV/00027006:_____/21:10174364 - isvavai.cz</a>

Výsledek na webu

<a href="https://op.europa.eu/en/publication-detail/-/publication/7c05be4a-00e3-11ec-8f47-01aa75ed71a1/language-en" target="_blank" >https://op.europa.eu/en/publication-detail/-/publication/7c05be4a-00e3-11ec-8f47-01aa75ed71a1/language-en</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2760/243914" target="_blank" >10.2760/243914</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Guidance document on multiplex real-time PCR methods

Popis výsledku v původním jazyce

Polymerase Chain Reaction (PCR) is considered the gold standard technique for the detection and quantification of Genetically Modified (GM) food, feed and seeds. The combination of two or more PCR methods in a single reaction tube is referred to as multiplex PCR and is a powerful approach already adopted by many testing laboratories. Multiplex PCR can address the need for increased and more efficient testing for the presence of GMOs in food, feed or seeds and for the quantification of GM events. Although real-time PCR has been used for GMO testing for many years as well as for research, forensic or diagnostic purposes, a comprehensive guidance on the development and implementation of multiplex PCR is currently not available. This document has been developed by experts from the European Network of GMO Laboratories (ENGL). It provides background information on the technical aspects of multiplex PCR (mpPCR), including information on instruments, fluorescent dye selection and reaction controls. It gives guidance on how to implement a previously validated mpPCR method (i.e. method verification), as well as how to develop and validate a new mpPCR method. Guidance on criteria for the acceptance of methods, examples of how to assess these criteria and troubleshooting tips are given. Finally, a list of currently used mpPCR methods is included.

Název v anglickém jazyce

Guidance document on multiplex real-time PCR methods

Popis výsledku anglicky

Polymerase Chain Reaction (PCR) is considered the gold standard technique for the detection and quantification of Genetically Modified (GM) food, feed and seeds. The combination of two or more PCR methods in a single reaction tube is referred to as multiplex PCR and is a powerful approach already adopted by many testing laboratories. Multiplex PCR can address the need for increased and more efficient testing for the presence of GMOs in food, feed or seeds and for the quantification of GM events. Although real-time PCR has been used for GMO testing for many years as well as for research, forensic or diagnostic purposes, a comprehensive guidance on the development and implementation of multiplex PCR is currently not available. This document has been developed by experts from the European Network of GMO Laboratories (ENGL). It provides background information on the technical aspects of multiplex PCR (mpPCR), including information on instruments, fluorescent dye selection and reaction controls. It gives guidance on how to implement a previously validated mpPCR method (i.e. method verification), as well as how to develop and validate a new mpPCR method. Guidance on criteria for the acceptance of methods, examples of how to assess these criteria and troubleshooting tips are given. Finally, a list of currently used mpPCR methods is included.

Druh

B - Odborná kniha

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

ISBN

978-92-76-37820-4

Počet stran knihy

62

Název nakladatele

Publications Office of the European Union

Místo vydání

Luxembourg

Kód UT WoS knihy

—