A chromosome-level genome of the booklouse, Liposcelis brunnea, provides insight into louse evolution and environmental stress adaptation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F22%3A10175266" target="_blank" >RIV/00027006:_____/22:10175266 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/22:91355

Výsledek na webu

<a href="https://scholar.google.com/scholar_url?url=https://academic.oup.com/gigascience/article-pdf/doi/10.1093/gigascience/giac062/45025325/giac062.pdf&hl=cs&sa=T&oi=ucasa&ct=ufr&ei=BHBiY6LGCYKEmgHSh7CYBQ&scisig=AAGBfm0qVBRdjvSj4J67EmIG7mjws9wjhQ" target="_blank" >https://scholar.google.com/scholar_url?url=https://academic.oup.com/gigascience/article-pdf/doi/10.1093/gigascience/giac062/45025325/giac062.pdf&hl=cs&sa=T&oi=ucasa&ct=ufr&ei=BHBiY6LGCYKEmgHSh7CYBQ&scisig=AAGBfm0qVBRdjvSj4J67EmIG7mjws9wjhQ</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/gigascience/giac062" target="_blank" >10.1093/gigascience/giac062</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A chromosome-level genome of the booklouse, Liposcelis brunnea, provides insight into louse evolution and environmental stress adaptation

Popis výsledku v původním jazyce

Background Booklice (psocids) in the genus Liposcelis (Psocoptera: Liposcelididae) are a group of important storage pests, found in libraries, grain storages, and food-processing facilities. Booklice are able to survive under heat treatment and typically possess high resistance to common fumigant insecticides, hence posing a threat to storage security worldwide.Results We assembled the genome of the booklouse, L. brunnea, the first genome reported in Psocoptera, using PacBio long-read sequencing, Illumina sequencing, and chromatin conformation capture (Hi-C) methods. After assembly, polishing, haplotype purging, and Hi-C scaffolding, we obtained 9 linkage groups (174.1 Mb in total) ranging from 12.1 Mb to 27.6 Mb (N50: 19.7 Mb), with the BUSCO completeness at 98.9%. In total, 15,543 genes were predicted by the Maker pipeline. Gene family analyses indicated the sensing-related gene families (OBP and OR) and the resistance-related gene families (ABC, EST, GST, UGT, and P450) expanded significantly in L. brunnea compared with those of their closest relatives (2 parasitic lice). Based on transcriptomic analysis, we found that the CYP4 subfamily from the P450 gene family functioned during phosphine fumigation; HSP genes, particularly those from the HSP70 subfamily, were upregulated significantly under high temperatures.Conclusions We present a chromosome-level genome assembly of L. brunnea, the first genome reported for the order Psocoptera. Our analyses provide new insights into the gene family evolution of the louse clade and the transcriptomic responses of booklice to environmental stresses.

Název v anglickém jazyce

A chromosome-level genome of the booklouse, Liposcelis brunnea, provides insight into louse evolution and environmental stress adaptation

Popis výsledku anglicky

Background Booklice (psocids) in the genus Liposcelis (Psocoptera: Liposcelididae) are a group of important storage pests, found in libraries, grain storages, and food-processing facilities. Booklice are able to survive under heat treatment and typically possess high resistance to common fumigant insecticides, hence posing a threat to storage security worldwide.Results We assembled the genome of the booklouse, L. brunnea, the first genome reported in Psocoptera, using PacBio long-read sequencing, Illumina sequencing, and chromatin conformation capture (Hi-C) methods. After assembly, polishing, haplotype purging, and Hi-C scaffolding, we obtained 9 linkage groups (174.1 Mb in total) ranging from 12.1 Mb to 27.6 Mb (N50: 19.7 Mb), with the BUSCO completeness at 98.9%. In total, 15,543 genes were predicted by the Maker pipeline. Gene family analyses indicated the sensing-related gene families (OBP and OR) and the resistance-related gene families (ABC, EST, GST, UGT, and P450) expanded significantly in L. brunnea compared with those of their closest relatives (2 parasitic lice). Based on transcriptomic analysis, we found that the CYP4 subfamily from the P450 gene family functioned during phosphine fumigation; HSP genes, particularly those from the HSP70 subfamily, were upregulated significantly under high temperatures.Conclusions We present a chromosome-level genome assembly of L. brunnea, the first genome reported for the order Psocoptera. Our analyses provide new insights into the gene family evolution of the louse clade and the transcriptomic responses of booklice to environmental stresses.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/LTACH19029" target="_blank" >LTACH19029: Invazivní mechanismy hospodářsky významných skladištních hmyzích škůdců ohrožujících čínský a evropský mezinárodní obchod a systémy fytosanitární techniky pro jejich omezování</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

GigaScience

ISSN

2047-217X

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

2022

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1-10

Kód UT WoS článku

000846739000076

EID výsledku v databázi Scopus

2-s2.0-85134556725