Evaluation of genes associated with resistance to Barley Yellow Dwarf Virus infection and impact of climate change on virus-vector interactions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F22%3A10175469" target="_blank" >RIV/00027006:_____/22:10175469 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of genes associated with resistance to Barley Yellow Dwarf Virus infection and impact of climate change on virus-vector interactions

Popis výsledku v původním jazyce

Barley yellow dwarf virus (BYDV) complex causes one of the most economically important virus diseases in cereals worldwide. We have found several genotypes of barley and wheat that show a high level of resistance to BYDV (Jarošová et al., 2013; Bioni et al., 2016). Further RNA-seq analysis and expression of miRNAs revealed differential expression of numerous miRNAs associated with resistance to BYDV (Jarošová et al., 2020). Analysis of the expression of selected miRNAs-target genes such as the NBS, CC-NBS-LRR and RLK gene classes revealed a uniform response to BYDV in both barley and wheat genotypes regardless of their resistance level or post-inoculation stage, with the exception of RLK genes. RLK genes were up-regulated in the susceptible genotype at both early and late post-infection stages, in contrast, they were down-regulated in the resistant genotype at early and late post-infection stages in barley and wheat, respectively. The expression of casein kinase, protein kinase and protein phosphatase domains in response to BYDV infection showed a differential response with respect to the level of resistance and between barley and wheat. The expression of RLK genes could therefore be an effective tool to assess the resistance profile of cereals to BYDV. The study of the impact of the virus and its aphid vector Rhopalosiphum padi on changing climatic conditions has shown that: i) higher temperatures lead to higher BYDV titre, severe disease symptoms, shorter life cycles and higher aphid fecundity. As a result, the plant is more attractive to aphid vectors and the efficiency of virus transmission is high; ii) elevated CO2 has a similar effect and contributes to a higher rate of virus transmission by aphids in a shorter period than elevated temperature; iii) elevated temperature and CO2 generally have less negative effects on the resistant genotypes than on the susceptible ones.

Název v anglickém jazyce

Evaluation of genes associated with resistance to Barley Yellow Dwarf Virus infection and impact of climate change on virus-vector interactions

Popis výsledku anglicky

Barley yellow dwarf virus (BYDV) complex causes one of the most economically important virus diseases in cereals worldwide. We have found several genotypes of barley and wheat that show a high level of resistance to BYDV (Jarošová et al., 2013; Bioni et al., 2016). Further RNA-seq analysis and expression of miRNAs revealed differential expression of numerous miRNAs associated with resistance to BYDV (Jarošová et al., 2020). Analysis of the expression of selected miRNAs-target genes such as the NBS, CC-NBS-LRR and RLK gene classes revealed a uniform response to BYDV in both barley and wheat genotypes regardless of their resistance level or post-inoculation stage, with the exception of RLK genes. RLK genes were up-regulated in the susceptible genotype at both early and late post-infection stages, in contrast, they were down-regulated in the resistant genotype at early and late post-infection stages in barley and wheat, respectively. The expression of casein kinase, protein kinase and protein phosphatase domains in response to BYDV infection showed a differential response with respect to the level of resistance and between barley and wheat. The expression of RLK genes could therefore be an effective tool to assess the resistance profile of cereals to BYDV. The study of the impact of the virus and its aphid vector Rhopalosiphum padi on changing climatic conditions has shown that: i) higher temperatures lead to higher BYDV titre, severe disease symptoms, shorter life cycles and higher aphid fecundity. As a result, the plant is more attractive to aphid vectors and the efficiency of virus transmission is high; ii) elevated CO2 has a similar effect and contributes to a higher rate of virus transmission by aphids in a shorter period than elevated temperature; iii) elevated temperature and CO2 generally have less negative effects on the resistant genotypes than on the susceptible ones.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů