Detection and quantification of Puccinia graminis and Piccinia tricina in host plant tissue by real-time PCR
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F22%3A10175506" target="_blank" >RIV/00027006:_____/22:10175506 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Detection and quantification of Puccinia graminis and Piccinia tricina in host plant tissue by real-time PCR
Popis výsledku v původním jazyce
Leaf rust, caused by Puccinia triticina Eriks., is wheat's most common rust disease (Triticum aestivum L.) in more worldwide regions. Puccinia graminis Pers. is one of the other most devastating diseases of cereal crops grown worldwide, often resulting in significant yield losses. This work aims to determine the quantity of the given rust in training populations of Triticum aestivum cultivars bred for resistance to rusts.Tubulins are well conserved in eukaryotes and are molecular targets of MBC fungicides, effectively controlling many plant diseases caused by ascomycete fungi. Therefore, the B-tubulin gene was selected as a molecular marker, and primers and probes were developed for it. The symptomatic evaluation was performed using image analysis and RT-PCR quantified pathogen content.By applying genomic selection, the material with the best combination of the observed traits associated with resistance to these rusts in the population will be selected.
Název v anglickém jazyce
Detection and quantification of Puccinia graminis and Piccinia tricina in host plant tissue by real-time PCR
Popis výsledku anglicky
Leaf rust, caused by Puccinia triticina Eriks., is wheat's most common rust disease (Triticum aestivum L.) in more worldwide regions. Puccinia graminis Pers. is one of the other most devastating diseases of cereal crops grown worldwide, often resulting in significant yield losses. This work aims to determine the quantity of the given rust in training populations of Triticum aestivum cultivars bred for resistance to rusts.Tubulins are well conserved in eukaryotes and are molecular targets of MBC fungicides, effectively controlling many plant diseases caused by ascomycete fungi. Therefore, the B-tubulin gene was selected as a molecular marker, and primers and probes were developed for it. The symptomatic evaluation was performed using image analysis and RT-PCR quantified pathogen content.By applying genomic selection, the material with the best combination of the observed traits associated with resistance to these rusts in the population will be selected.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů