Assessment and quantification of new metabolites in quinoa (Chenopodium quinoa) using UHPLC-Q-Orbitrap mass spectrometer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F22%3A10175523" target="_blank" >RIV/00027006:_____/22:10175523 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Assessment and quantification of new metabolites in quinoa (Chenopodium quinoa) using UHPLC-Q-Orbitrap mass spectrometer

Popis výsledku v původním jazyce

In this study, 56 various quinoa genotypes grown in the climatic conditions of the Czech Republic during the year 2021 were subjected to metabolomic analyses using UHPLC-Q-Orbitrap high-resolution tandem mass spectrometer. Obtained data were processed in Xcalibur Quan Browser and Compound Discoverer software and compared to available scientific literature. As a result, eight flavonoids, two phenolic compounds, and one phenolic acid were detected and quantified in quinoa samples. The majority of them has never been quantified in quinoa before. The concentration of all 11 compounds was compared among red- and yellow-colored seed samples of the same genotype. Red seeded genotypes had higher mean concentration rates of analyzed metabolites than yellow seeded genotypes. Chemicals identified in absolutely all quinoa samples were isoquercetin, naringenin, pinocembrin, emodin, isorhamnetin from flavonoid group, and salicylic acid as a phenolic compound. Two chemicals, namely 4-hydroxybenzaldehyde (0.576-4.233 μg/g DW) and N-feruloyloctopamine (0.092-4.993 μg/g DW) have never been identified or quantified in quinoa before. Both are additionally the first compounds found in the genus Chenopodium and the family Chenopodiaceae. These findings showed that quinoa metabolomics is a potential area for further investigations since the variability of chemical compounds has not been fully explored yet

Název v anglickém jazyce

Assessment and quantification of new metabolites in quinoa (Chenopodium quinoa) using UHPLC-Q-Orbitrap mass spectrometer

Popis výsledku anglicky

In this study, 56 various quinoa genotypes grown in the climatic conditions of the Czech Republic during the year 2021 were subjected to metabolomic analyses using UHPLC-Q-Orbitrap high-resolution tandem mass spectrometer. Obtained data were processed in Xcalibur Quan Browser and Compound Discoverer software and compared to available scientific literature. As a result, eight flavonoids, two phenolic compounds, and one phenolic acid were detected and quantified in quinoa samples. The majority of them has never been quantified in quinoa before. The concentration of all 11 compounds was compared among red- and yellow-colored seed samples of the same genotype. Red seeded genotypes had higher mean concentration rates of analyzed metabolites than yellow seeded genotypes. Chemicals identified in absolutely all quinoa samples were isoquercetin, naringenin, pinocembrin, emodin, isorhamnetin from flavonoid group, and salicylic acid as a phenolic compound. Two chemicals, namely 4-hydroxybenzaldehyde (0.576-4.233 μg/g DW) and N-feruloyloctopamine (0.092-4.993 μg/g DW) have never been identified or quantified in quinoa before. Both are additionally the first compounds found in the genus Chenopodium and the family Chenopodiaceae. These findings showed that quinoa metabolomics is a potential area for further investigations since the variability of chemical compounds has not been fully explored yet

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů