Assessment of the Cadmium and Copper Phytoremediation Potential of the Lobularia maritima Thioredoxin 2 Gene Using Genetically Engineered Tobacco
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F23%3A10176203" target="_blank" >RIV/00027006:_____/23:10176203 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61989592:15640/23:73620300
Výsledek na webu
<a href="https://www.mdpi.com/2073-4395/13/2/399/pdf?version=1675766206" target="_blank" >https://www.mdpi.com/2073-4395/13/2/399/pdf?version=1675766206</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/agronomy13020399" target="_blank" >10.3390/agronomy13020399</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Assessment of the Cadmium and Copper Phytoremediation Potential of the Lobularia maritima Thioredoxin 2 Gene Using Genetically Engineered Tobacco
Popis výsledku v původním jazyce
Rapid global modernization, urbanization, and industrialization have accelerated the release of heavy metals, causing soil pollution. These highly noxious environmental pollutants induce oxidative stress in plants via stimulation of the production of reactive oxygen species (ROS). Thioredoxin (Trxs) is a highly conserved disulfide reductase that plays a crucial role in intracellular redox homeostasis in both eukaryotes and prokaryotes. Herein, the presence of heavy metals highly upregulated LmTrxh2 transcription in Lobularia maritima seedlings and its overexpression-conferred tolerance to Cd, Cu, Mn, and Zn in Saccharomyces cerevisiae. In addition, LmTrxh2-overexpressing tobacco plants had higher seedling survival rates than non-transgenic plants (NT), with enhanced root length and biomass production and reduced ROS accumulation, following Cd and Cu stress. These plants also accumulated more Cd, Cu, and Mn than the NT plants. Moreover, LmTrxh2 overexpression stimulated the transcription of genes encoding metallothioneins (Met-1, Met-2, Met-3, and Met-4), a copper transport protein, a Snakin/GASA protein (Snakin-2), and ROS-scavenging enzymes (SOD, APX1, and CAT), which might contribute to heavy metal tolerance in tobacco plants. These results suggest that LmTrxh2 overexpression helps to improve heavy metal tolerance by stimulating antioxidant capacities and the expression of several stress-responsive genes in plants.
Název v anglickém jazyce
Assessment of the Cadmium and Copper Phytoremediation Potential of the Lobularia maritima Thioredoxin 2 Gene Using Genetically Engineered Tobacco
Popis výsledku anglicky
Rapid global modernization, urbanization, and industrialization have accelerated the release of heavy metals, causing soil pollution. These highly noxious environmental pollutants induce oxidative stress in plants via stimulation of the production of reactive oxygen species (ROS). Thioredoxin (Trxs) is a highly conserved disulfide reductase that plays a crucial role in intracellular redox homeostasis in both eukaryotes and prokaryotes. Herein, the presence of heavy metals highly upregulated LmTrxh2 transcription in Lobularia maritima seedlings and its overexpression-conferred tolerance to Cd, Cu, Mn, and Zn in Saccharomyces cerevisiae. In addition, LmTrxh2-overexpressing tobacco plants had higher seedling survival rates than non-transgenic plants (NT), with enhanced root length and biomass production and reduced ROS accumulation, following Cd and Cu stress. These plants also accumulated more Cd, Cu, and Mn than the NT plants. Moreover, LmTrxh2 overexpression stimulated the transcription of genes encoding metallothioneins (Met-1, Met-2, Met-3, and Met-4), a copper transport protein, a Snakin/GASA protein (Snakin-2), and ROS-scavenging enzymes (SOD, APX1, and CAT), which might contribute to heavy metal tolerance in tobacco plants. These results suggest that LmTrxh2 overexpression helps to improve heavy metal tolerance by stimulating antioxidant capacities and the expression of several stress-responsive genes in plants.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Návaznosti výsledku
Projekt
<a href="/cs/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Rostliny jako prostředek udržitelného globálního rozvoje</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Agronomy-Basel
ISSN
2073-4395
e-ISSN
—
Svazek periodika
13
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
14
Strana od-do
399
Kód UT WoS článku
000938129600001
EID výsledku v databázi Scopus
2-s2.0-85149124177