EP05 Extraction of rosmarinic acid from Ocimum sanctum using Nades

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F23%3A10176581" target="_blank" >RIV/00027006:_____/23:10176581 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

EP05 Extraction of rosmarinic acid from Ocimum sanctum using Nades

Popis výsledku v původním jazyce

Rosmarinic acid (RA) and its derivatives exhibit biological actions against various diseases suchas cancer, diabetes, cardiovascular disease, neurodegenerative and inflammatory disorders. The most used conventional techniques for RA extraction from plants are Soxhlet extraction, maceration and new techniques have been introduced such as ultrasound-assisted extraction, microwave-assisted extraction, and supercritical fluid extraction using mainly organic solvents, e.g., ethanol, or acidified ethanol. Due to legislation restriction regarding ethanol, there is a demand for a solvent meeting the requirements (food industry). The solution could be Nades (Natural Deep Eutectic Solvents), which are very closed to the plants physiology. We studied mixtures of choline chloride:glucose, choline chloride:propane-1,2 diol and citric acid:glucose as examples of RA extraction using Nades. Dried, and ground basil leaves were mixed with the prepared Nades and extracted at laboratory temperature with constant stirring, then the mixture was analysed by HPLC. Extraction with citric acid:glucose mixtures is more selective and provides comparable or even higher yields of RA compared to methanol extraction.

Název v anglickém jazyce

EP05 Extraction of rosmarinic acid from Ocimum sanctum using Nades

Popis výsledku anglicky

Rosmarinic acid (RA) and its derivatives exhibit biological actions against various diseases suchas cancer, diabetes, cardiovascular disease, neurodegenerative and inflammatory disorders. The most used conventional techniques for RA extraction from plants are Soxhlet extraction, maceration and new techniques have been introduced such as ultrasound-assisted extraction, microwave-assisted extraction, and supercritical fluid extraction using mainly organic solvents, e.g., ethanol, or acidified ethanol. Due to legislation restriction regarding ethanol, there is a demand for a solvent meeting the requirements (food industry). The solution could be Nades (Natural Deep Eutectic Solvents), which are very closed to the plants physiology. We studied mixtures of choline chloride:glucose, choline chloride:propane-1,2 diol and citric acid:glucose as examples of RA extraction using Nades. Dried, and ground basil leaves were mixed with the prepared Nades and extracted at laboratory temperature with constant stirring, then the mixture was analysed by HPLC. Extraction with citric acid:glucose mixtures is more selective and provides comparable or even higher yields of RA compared to methanol extraction.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK1910103" target="_blank" >QK1910103: České bylinky pro nové potraviny podporující zdraví populace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů