Water state of apricot stem cuttings during dehydration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F23%3A10176633" target="_blank" >RIV/00027006:_____/23:10176633 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Water state of apricot stem cuttings during dehydration

Popis výsledku v původním jazyce

Many cryopreservation protocols require dehydration before freezing to prevent cryodamageassociated with water crystallization. The purpose of this study was to evaluate changes in water statein tissues of apricot stem cuttings at different dehydration levels. The water activity and water contentin control apricot nodal segments of Sophinka, Candela, and Leala varieties were not significantlydifferent and were in the range of 0.96 - 0.97 and 48.2 - 49.6%, respectively. In the temperaturerange from -90 to +30 oC, calorimetric studies of apricot dormant buds during cooling showed, thatthe crystallization event occurred at two separate temperatures. The onset temperature of the maincrystallization peak for the apricot buds was from -6.5 to -9.3 oC. The percentage of crystallized waterwas 35.2 - 36.9 % and was not significantly different for all varieties. The second peak ofcrystallization was recorded from -37.7 to -41.3 oC and the percentage of crystallized water was lessthan 1% at this temperature range. During heating of frozen buds, one main melting peak wasidentified with the onset temperature from -4.6 to -7.9 oC. After 11 days of dehydration, the watercontent, water activity and percentage of crystallized water, depending on the variety, decreased to26.3 - 30.1 %, 0.84 - 0.88, and 2.9 - 18.1 %, respectively. The rate of decrease in water content,water activity and percentage of crystallized water for the Leala variety was higher than for othervarieties. It can be concluded that thermal characterization and analysis of water activity in stemcuttings and buds provides sufficiently complete information on the water status of the plant tissuefor cryopreservation.

Název v anglickém jazyce

Water state of apricot stem cuttings during dehydration

Popis výsledku anglicky

Many cryopreservation protocols require dehydration before freezing to prevent cryodamageassociated with water crystallization. The purpose of this study was to evaluate changes in water statein tissues of apricot stem cuttings at different dehydration levels. The water activity and water contentin control apricot nodal segments of Sophinka, Candela, and Leala varieties were not significantlydifferent and were in the range of 0.96 - 0.97 and 48.2 - 49.6%, respectively. In the temperaturerange from -90 to +30 oC, calorimetric studies of apricot dormant buds during cooling showed, thatthe crystallization event occurred at two separate temperatures. The onset temperature of the maincrystallization peak for the apricot buds was from -6.5 to -9.3 oC. The percentage of crystallized waterwas 35.2 - 36.9 % and was not significantly different for all varieties. The second peak ofcrystallization was recorded from -37.7 to -41.3 oC and the percentage of crystallized water was lessthan 1% at this temperature range. During heating of frozen buds, one main melting peak wasidentified with the onset temperature from -4.6 to -7.9 oC. After 11 days of dehydration, the watercontent, water activity and percentage of crystallized water, depending on the variety, decreased to26.3 - 30.1 %, 0.84 - 0.88, and 2.9 - 18.1 %, respectively. The rate of decrease in water content,water activity and percentage of crystallized water for the Leala variety was higher than for othervarieties. It can be concluded that thermal characterization and analysis of water activity in stemcuttings and buds provides sufficiently complete information on the water status of the plant tissuefor cryopreservation.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

R - Projekt Ramcoveho programu EK

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů