3D PRINTING WITH ESSENTIAL OIL IN HYDROPHILIC SUPPORT GEL

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F23%3A10176643" target="_blank" >RIV/00027006:_____/23:10176643 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

3D PRINTING WITH ESSENTIAL OIL IN HYDROPHILIC SUPPORT GEL

Popis výsledku v původním jazyce

In recent years, much attention has been paid to the characterization and creation ofnew bioinks, as it is believed that this is one of the main reasons for the slowdown in thedevelopment of the 3D bioprinting field. Therefore, the purpose of this study is toevaluate the possibility of using essential oils as bioinks of plant origin.To create scaffolds, a Coolness 3D printer (IPC&amp;C, Ukraine) was used, and aninfusion pump was used as a dosing system for bioink. The 3D print models were createdusing FreeCAD software. All STL-files were prepared for printing using the free Curaslicing software, with layer thicknesses of 450 µm to generate G-code for 3D printer. Theprinting process was controlled using the open source Pronterface software package. Theessential oil of Oríganum vulgare, obtained by extraction with liquefied gases, was usedas bioink. Printing was carried out in a hydrophilic support gel consisting of 1.2%Carbopol 940 and 0.04% bromothymol blue. To increase the viscosity of Carbopol 940, a10 M NaOH solution was used. The shape fidelity and characterization of filaments of the3D scaffolds were evaluated according to the protocol described by Schwab et al. (2020).Three-dimensional constructions were obtained printed with the essential oil ofOríganum vulgáre in the hydrophilic gel. In all the cases studied, the obtained filamentswere heterogeneous. During the study, it was found that an extruder with a hole diameterof 0.583 +- 0.05 mm is most suitable for printing with essential oil. When printingstructures with an extruder with this hole, a decrease in an uneven index of printing alongall the axes under study is observed. Thus, the uneven index of printing along the Z axiswas 123.8%, while an increase in the diameter of the extruder hole by 2.4 times leads to aproportional increase in the unevenness parameter. Reducing the extruder diameter below0.583 results in uneven extrusion of the bioink. We have shown that the optimal bioinkextrusion speed is 100 mm/s, while the printing unevenness along the X, Y, Z axes is94.66%, 96.47%, and 123.48, respectively. For all printed structures, the value of the poreprintability coefficient lies in the range from 0.92 to 1.00, which in turn indicates a goodgeometry of the printed pores.The conducted research indicates the possibility of using Oríganum vulgare essentialoil as a bioink for 3D printing in a hydrophilic support gel.

Název v anglickém jazyce

3D PRINTING WITH ESSENTIAL OIL IN HYDROPHILIC SUPPORT GEL

Popis výsledku anglicky

In recent years, much attention has been paid to the characterization and creation ofnew bioinks, as it is believed that this is one of the main reasons for the slowdown in thedevelopment of the 3D bioprinting field. Therefore, the purpose of this study is toevaluate the possibility of using essential oils as bioinks of plant origin.To create scaffolds, a Coolness 3D printer (IPC&amp;C, Ukraine) was used, and aninfusion pump was used as a dosing system for bioink. The 3D print models were createdusing FreeCAD software. All STL-files were prepared for printing using the free Curaslicing software, with layer thicknesses of 450 µm to generate G-code for 3D printer. Theprinting process was controlled using the open source Pronterface software package. Theessential oil of Oríganum vulgare, obtained by extraction with liquefied gases, was usedas bioink. Printing was carried out in a hydrophilic support gel consisting of 1.2%Carbopol 940 and 0.04% bromothymol blue. To increase the viscosity of Carbopol 940, a10 M NaOH solution was used. The shape fidelity and characterization of filaments of the3D scaffolds were evaluated according to the protocol described by Schwab et al. (2020).Three-dimensional constructions were obtained printed with the essential oil ofOríganum vulgáre in the hydrophilic gel. In all the cases studied, the obtained filamentswere heterogeneous. During the study, it was found that an extruder with a hole diameterof 0.583 +- 0.05 mm is most suitable for printing with essential oil. When printingstructures with an extruder with this hole, a decrease in an uneven index of printing alongall the axes under study is observed. Thus, the uneven index of printing along the Z axiswas 123.8%, while an increase in the diameter of the extruder hole by 2.4 times leads to aproportional increase in the unevenness parameter. Reducing the extruder diameter below0.583 results in uneven extrusion of the bioink. We have shown that the optimal bioinkextrusion speed is 100 mm/s, while the printing unevenness along the X, Y, Z axes is94.66%, 96.47%, and 123.48, respectively. For all printed structures, the value of the poreprintability coefficient lies in the range from 0.92 to 1.00, which in turn indicates a goodgeometry of the printed pores.The conducted research indicates the possibility of using Oríganum vulgare essentialoil as a bioink for 3D printing in a hydrophilic support gel.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

—

Návaznosti

R - Projekt Ramcoveho programu EK

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů