Sequencing and recombination analysis of watermelon mosaic isolates from the czech republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F23%3A10176660" target="_blank" >RIV/00027006:_____/23:10176660 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Sequencing and recombination analysis of watermelon mosaic isolates from the czech republic

Popis výsledku v původním jazyce

Watermelon mosaic virus (WMV) is a virus from the genus potyvirus. It is one of the most economically important viral pathogens infecting cucurbits in the world. The occurrence of WMV in the Czech Republic was first reported by Svoboda in 2011 based on ELISA testing. Currently, and while attempting to update the viral species frequency infecting cucurbits in the Czech Republic, five full-length coding region of WMV isolates were obtained by high throughput sequencing and also by Sanger sequencing together with 26 more isolates sequenced in the coat protein coding region. The screening for recombination events in the complete dataset revealed the presence of eighty independent recombination events. The frequency of recombinant WMV isolates reached 96.24%. Therefore, the watermelon mosaic virus showed to have the highest number of recombinants compared to any other potyviruses.

Název v anglickém jazyce

Sequencing and recombination analysis of watermelon mosaic isolates from the czech republic

Popis výsledku anglicky

Watermelon mosaic virus (WMV) is a virus from the genus potyvirus. It is one of the most economically important viral pathogens infecting cucurbits in the world. The occurrence of WMV in the Czech Republic was first reported by Svoboda in 2011 based on ELISA testing. Currently, and while attempting to update the viral species frequency infecting cucurbits in the Czech Republic, five full-length coding region of WMV isolates were obtained by high throughput sequencing and also by Sanger sequencing together with 26 more isolates sequenced in the coat protein coding region. The screening for recombination events in the complete dataset revealed the presence of eighty independent recombination events. The frequency of recombinant WMV isolates reached 96.24%. Therefore, the watermelon mosaic virus showed to have the highest number of recombinants compared to any other potyviruses.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů