Applicability of microsatellite markers for identification of new sources of resistance to downy mildew (Hyaloperonospora parasitica) in cultivars of various Brassica oleracea crops and several wild Brassica species deposited in the genebank collections

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F23%3A10176686" target="_blank" >RIV/00027006:_____/23:10176686 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Applicability of microsatellite markers for identification of new sources of resistance to downy mildew (Hyaloperonospora parasitica) in cultivars of various Brassica oleracea crops and several wild Brassica species deposited in the genebank collections

Popis výsledku v původním jazyce

Breeding for disease resistance is one of the primary objectives in most crop-breeding programs. Over the last decade, molecular marker technology has been widely integrated, including molecular marker-assisted selection and breeding. The present study aimed to identify resistant materials against downy mildew (Hyaloperonospora parasitica) among 274 mainly European Genebank accessions of various Brassica oleracea crops (broccoli, Brussels sprouts, cabbage, cauliflower, kale, and kohlrabi), as well as several wild Brassica species. The study tested the applicability of microsatellite (SSR) markers associated with loci Pp523 (CB10028 and CB10139), Ppa207 (BoGMS0900 and BoGMS0486), Ppa 3 (BoGMS0624), and the BoGS-ELONG gene (BODM1) linked to resistance against downy mildew in Brassica species. These markers were subsequently applied to 309 individual plants from 112 selected Brassica species accessions, exhibiting different levels of resistance to five isolates of Hyaloperenospora parasitica at the cotyledon stage. The markers amplified 29 alleles across the analyzed accessions inconsistently, even when plants of standard genotypes were used.Nevertheless, marker CB10028 demonstrated the highest consistency, indicating a 158 bp allele for resistant cauliflower and a 149 bp allele for susceptible broccoli, kale, and kohlrabi. Another relatively consistent marker, BoGMS900, indicated 146-148 bp alleles for resistant broccoli and cauliflower and a 132 bp allele for susceptible broccoli, Brussels sprouts, and wild species. These results suggest the effective participation of Pp523 and Ppa207 in the resistance of seedling Brassica oleracea crops.

Název v anglickém jazyce

Applicability of microsatellite markers for identification of new sources of resistance to downy mildew (Hyaloperonospora parasitica) in cultivars of various Brassica oleracea crops and several wild Brassica species deposited in the genebank collections

Popis výsledku anglicky

Breeding for disease resistance is one of the primary objectives in most crop-breeding programs. Over the last decade, molecular marker technology has been widely integrated, including molecular marker-assisted selection and breeding. The present study aimed to identify resistant materials against downy mildew (Hyaloperonospora parasitica) among 274 mainly European Genebank accessions of various Brassica oleracea crops (broccoli, Brussels sprouts, cabbage, cauliflower, kale, and kohlrabi), as well as several wild Brassica species. The study tested the applicability of microsatellite (SSR) markers associated with loci Pp523 (CB10028 and CB10139), Ppa207 (BoGMS0900 and BoGMS0486), Ppa 3 (BoGMS0624), and the BoGS-ELONG gene (BODM1) linked to resistance against downy mildew in Brassica species. These markers were subsequently applied to 309 individual plants from 112 selected Brassica species accessions, exhibiting different levels of resistance to five isolates of Hyaloperenospora parasitica at the cotyledon stage. The markers amplified 29 alleles across the analyzed accessions inconsistently, even when plants of standard genotypes were used.Nevertheless, marker CB10028 demonstrated the highest consistency, indicating a 158 bp allele for resistant cauliflower and a 149 bp allele for susceptible broccoli, kale, and kohlrabi. Another relatively consistent marker, BoGMS900, indicated 146-148 bp alleles for resistant broccoli and cauliflower and a 132 bp allele for susceptible broccoli, Brussels sprouts, and wild species. These results suggest the effective participation of Pp523 and Ppa207 in the resistance of seedling Brassica oleracea crops.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

R - Projekt Ramcoveho programu EK

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů