Utilization of new sterilization methods for introduction into in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F24%3A10177745" target="_blank" >RIV/00027006:_____/24:10177745 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Utilization of new sterilization methods for introduction into in vitro

Popis výsledku v původním jazyce

Introducing woody crops into in vitro conditions poses challenges due to potential contamination from a wide range of pathogens on their surfaces. This study aimed to assess the feasibility of using dormant buds from selected fruit tree species for in vitro initiation and subsequent multiplication in bioreactors utilizing a temporary immersion system (TIS). Experiments were conducted with specific raspberry varieties cultivated in the hotbed of the Crop Research Institute in Prague. The in vitro regeneration method tested involved surface sterilization using chlorine-based disinfectants. Initially, uninodal bud segments underwent washing with tap water, treatment with commercial bleach, ethanol application, and final rinsing with sterilized water. Buds with approximately 1 cm of the attached twig were then placed on a semisolid modified MS medium containing 30 g L-1 sucrose, 1 mg L-1 BAP, 0.1 mg L-1 IBA, and 7 g L-1 agar. Newly sprouted shoots were subsequently transferred to bioreactors with the same medium composition, excluding agar. Growth of in vitro cultures and the response of other fruit tree species in the bioreactor system were evaluated. The proposed method demonstrates potential for use in the in vitro cultivation system of selected fruit tree species.

Název v anglickém jazyce

Utilization of new sterilization methods for introduction into in vitro

Popis výsledku anglicky

Introducing woody crops into in vitro conditions poses challenges due to potential contamination from a wide range of pathogens on their surfaces. This study aimed to assess the feasibility of using dormant buds from selected fruit tree species for in vitro initiation and subsequent multiplication in bioreactors utilizing a temporary immersion system (TIS). Experiments were conducted with specific raspberry varieties cultivated in the hotbed of the Crop Research Institute in Prague. The in vitro regeneration method tested involved surface sterilization using chlorine-based disinfectants. Initially, uninodal bud segments underwent washing with tap water, treatment with commercial bleach, ethanol application, and final rinsing with sterilized water. Buds with approximately 1 cm of the attached twig were then placed on a semisolid modified MS medium containing 30 g L-1 sucrose, 1 mg L-1 BAP, 0.1 mg L-1 IBA, and 7 g L-1 agar. Newly sprouted shoots were subsequently transferred to bioreactors with the same medium composition, excluding agar. Growth of in vitro cultures and the response of other fruit tree species in the bioreactor system were evaluated. The proposed method demonstrates potential for use in the in vitro cultivation system of selected fruit tree species.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů