Research on mixed sample test for the rapid detection of rice stripe virus and southern rice black-streaked dwarf virus in vector

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10177941" target="_blank" >RIV/00027006:_____/25:10177941 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/journal/virology/vol/604/suppl/C" target="_blank" >https://www.sciencedirect.com/journal/virology/vol/604/suppl/C</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.virol.2025.110425" target="_blank" >10.1016/j.virol.2025.110425</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Research on mixed sample test for the rapid detection of rice stripe virus and southern rice black-streaked dwarf virus in vector

Popis výsledku v původním jazyce

Rice is a staple food in East and Southeast Asian countries, rice disease surveillance and warning is particularly important. Rice stripe disease and southern rice black-streaked dwarf virus disease are the insect-borne viral diseases that easily break out and quickly become epidemic. It is important to improve the efficiency of the virus detection method. In this work, a colloidal gold strip assay (CGSA) was used in mixed samples to detect rice stripe virus (RSV) and southern rice black-streaked dwarf virus (SRBSDV) in small brown planthoppers (SBPH) and white-backed planthoppers (WBPH), respectively. The results show that RSV could be detected in 9, 19 and 49 SBPHs mixed with a single positive SBPH sample, and SRBSDV could be detected in 9 WBPHs mixed with a single positive WBPH sample. The mixed sample detection technology presented in this article can significantly reduce the cost of detection and fulfil the requirements for large-scale, real-time virus surveillance in sentinel surveillance.

Název v anglickém jazyce

Research on mixed sample test for the rapid detection of rice stripe virus and southern rice black-streaked dwarf virus in vector

Popis výsledku anglicky

Rice is a staple food in East and Southeast Asian countries, rice disease surveillance and warning is particularly important. Rice stripe disease and southern rice black-streaked dwarf virus disease are the insect-borne viral diseases that easily break out and quickly become epidemic. It is important to improve the efficiency of the virus detection method. In this work, a colloidal gold strip assay (CGSA) was used in mixed samples to detect rice stripe virus (RSV) and southern rice black-streaked dwarf virus (SRBSDV) in small brown planthoppers (SBPH) and white-backed planthoppers (WBPH), respectively. The results show that RSV could be detected in 9, 19 and 49 SBPHs mixed with a single positive SBPH sample, and SRBSDV could be detected in 9 WBPHs mixed with a single positive WBPH sample. The mixed sample detection technology presented in this article can significantly reduce the cost of detection and fulfil the requirements for large-scale, real-time virus surveillance in sentinel surveillance.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

VIROLOGY

ISSN

0042-6822

e-ISSN

—

Svazek periodika

604

Číslo periodika v rámci svazku

MAR 2025

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

110425

Kód UT WoS článku

001421884600001

EID výsledku v databázi Scopus

2-s2.0-85216085371