Differences in the abundance and diversity of endosymbiotic bacteria drive host resistance of Philodromus cespitum, a dominant spider of central European orchards, to selected insecticides

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10177974" target="_blank" >RIV/00027006:_____/25:10177974 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/25:43927773

Výsledek na webu

<a href="https://www.sciencedirect.com/journal/journal-of-environmental-management/vol/373/suppl/C" target="_blank" >https://www.sciencedirect.com/journal/journal-of-environmental-management/vol/373/suppl/C</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jenvman.2024.123486" target="_blank" >10.1016/j.jenvman.2024.123486</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Differences in the abundance and diversity of endosymbiotic bacteria drive host resistance of Philodromus cespitum, a dominant spider of central European orchards, to selected insecticides

Popis výsledku v původním jazyce

The ability of tissue endosymbionts to degrade and detoxify agrochemicals is increasingly recognized as a mechanism supporting the survival of arthropods in agroecosystems. Therefore, tissue endosymbionts have the potential to drive insecticide resistance in agrobiont spiders, i.e., in major generalist predators and pest control agents within agroecosystems. We hypothesized that the abundance and diversity of the endosymbiotic bacteria of Philodromus cespitum, a philodromid spider dominating central European apple orchards, vary with regard to differences in predation capacity and drive host insecticide resistance. We provisioned P. cespitum with diets of varying protein and lipid content and topically exposed them to field-relevant doses of commonly used insecticides, namely Mospilan (acetamiprid), Movento (spirotetramat), Gondola (sulfoxaflor), Decis (deltamethrin), Coragen (chlorantraniliprole), and Benevia (cyantraniliprole). The analyses were based on 16S rDNA profiles from lysates of the cephalothorax and legs of the tested spiders. The application of Benevia, Mospilan, and Movento was partially lethal. The spiders that were resistant to the treatments with Benevia, Mospilan, or Movento were associated with the increased relative abundance of Mycoplasmatota by more than one order of magnitude. Additionally, the abundance of other bacteria differed in Mospilan-resistant and Mospilan-sensitive individuals. In contrast, the diet regimens were not associated with any major differences in the microbiome diversity nor the diversity of endosymbionts. Philodromus cespitum hosts assemblages with unexpectedly high beta diversity of endosymbionts. The OTU identified as the alpha proteobacterium endosymbiont of Coelostomidia zealandica was an obligate endosymbiont of the analyzed P. cespitum population. Wolbachia, Rickettsia, and Spiroplasma endosymbionts were also highly prevalent and differed in their responses to the applied treatments. In conclusion, differences in the abundance and diversity of endosymbiotic bacteria drove the resistance of the spider host to selected insecticides.

Název v anglickém jazyce

Differences in the abundance and diversity of endosymbiotic bacteria drive host resistance of Philodromus cespitum, a dominant spider of central European orchards, to selected insecticides

Popis výsledku anglicky

The ability of tissue endosymbionts to degrade and detoxify agrochemicals is increasingly recognized as a mechanism supporting the survival of arthropods in agroecosystems. Therefore, tissue endosymbionts have the potential to drive insecticide resistance in agrobiont spiders, i.e., in major generalist predators and pest control agents within agroecosystems. We hypothesized that the abundance and diversity of the endosymbiotic bacteria of Philodromus cespitum, a philodromid spider dominating central European apple orchards, vary with regard to differences in predation capacity and drive host insecticide resistance. We provisioned P. cespitum with diets of varying protein and lipid content and topically exposed them to field-relevant doses of commonly used insecticides, namely Mospilan (acetamiprid), Movento (spirotetramat), Gondola (sulfoxaflor), Decis (deltamethrin), Coragen (chlorantraniliprole), and Benevia (cyantraniliprole). The analyses were based on 16S rDNA profiles from lysates of the cephalothorax and legs of the tested spiders. The application of Benevia, Mospilan, and Movento was partially lethal. The spiders that were resistant to the treatments with Benevia, Mospilan, or Movento were associated with the increased relative abundance of Mycoplasmatota by more than one order of magnitude. Additionally, the abundance of other bacteria differed in Mospilan-resistant and Mospilan-sensitive individuals. In contrast, the diet regimens were not associated with any major differences in the microbiome diversity nor the diversity of endosymbionts. Philodromus cespitum hosts assemblages with unexpectedly high beta diversity of endosymbionts. The OTU identified as the alpha proteobacterium endosymbiont of Coelostomidia zealandica was an obligate endosymbiont of the analyzed P. cespitum population. Wolbachia, Rickettsia, and Spiroplasma endosymbionts were also highly prevalent and differed in their responses to the applied treatments. In conclusion, differences in the abundance and diversity of endosymbiotic bacteria drove the resistance of the spider host to selected insecticides.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/GA21-22765S" target="_blank" >GA21-22765S: Vliv endosymbiotických bakterií na predační kapacitu pavouků v agroekosystémech ošetřovaných pesticidy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF ENVIRONMENTAL MANAGEMENT

ISSN

0301-4797

e-ISSN

1095-8630

Svazek periodika

373

Číslo periodika v rámci svazku

JAN 2025

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

123486

Kód UT WoS článku

001370858900001

EID výsledku v databázi Scopus

2-s2.0-85210388356