Composite Polylactide/Polycaprolactone Foams with Hierarchical Porous Structure for Pre-Vascularized Tissue Engineering

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10178037" target="_blank" >RIV/00027006:_____/25:10178037 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/25:00619131 RIV/00216208:11110/25:10498182 RIV/60461373:22310/25:43931577 RIV/68407700:21220/25:00383038 a 3 dalších

Výsledek na webu

<a href="https://www.mdpi.com/1422-0067/26/7/2974/pdf?version=1742898015" target="_blank" >https://www.mdpi.com/1422-0067/26/7/2974/pdf?version=1742898015</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms26072974" target="_blank" >10.3390/ijms26072974</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Composite Polylactide/Polycaprolactone Foams with Hierarchical Porous Structure for Pre-Vascularized Tissue Engineering

Popis výsledku v původním jazyce

Modern tissue engineering requires not only degradable materials promoting cell growth and differentiation, but also vascularization of the engineered tissue. Porous polylactide/polycaprolactone (PLA/PCL, ratio 3/5) foam scaffolds were prepared by a combined porogen leaching and freeze-drying technique using NaCl (crystal size 250-500 mu m) and a water-soluble cellulose derivative (KlucelTM E; 10-100% w/w relative to the total PLA/PCL concentration) as porogens. Scanning electron microscopy, micro-CT, and Brunauer-Emmett-Teller analysis showed that all scaffolds contained a trimodal range of pore sizes, i.e., macropores (average diameter 298-539 mu m), micropores (100 nm to 10 mu m), and nanopores (mostly around 3.0 nm). All scaffolds had an open porosity of about 90%, and the pores were interconnected. The size of the macropores and the nanoporosity were higher in the scaffolds prepared with Klucel. Nanoporosity increased water uptake by the scaffolds, while macroporosity promoted cell ingrowth, which was most evident in scaffolds prepared with 25% Klucel. Human adipose-derived stem cells co-cultured with endothelial cells formed pre-vascular structures in the scaffolds, which was further enhanced in a dynamic cell culture system. The scaffolds are promising for the engineering of pre-vascularized soft tissues (relatively pliable 10% Klucel scaffolds) and hard tissues (mechanically stronger 25% and 50% Klucel scaffolds).

Název v anglickém jazyce

Composite Polylactide/Polycaprolactone Foams with Hierarchical Porous Structure for Pre-Vascularized Tissue Engineering

Popis výsledku anglicky

Modern tissue engineering requires not only degradable materials promoting cell growth and differentiation, but also vascularization of the engineered tissue. Porous polylactide/polycaprolactone (PLA/PCL, ratio 3/5) foam scaffolds were prepared by a combined porogen leaching and freeze-drying technique using NaCl (crystal size 250-500 mu m) and a water-soluble cellulose derivative (KlucelTM E; 10-100% w/w relative to the total PLA/PCL concentration) as porogens. Scanning electron microscopy, micro-CT, and Brunauer-Emmett-Teller analysis showed that all scaffolds contained a trimodal range of pore sizes, i.e., macropores (average diameter 298-539 mu m), micropores (100 nm to 10 mu m), and nanopores (mostly around 3.0 nm). All scaffolds had an open porosity of about 90%, and the pores were interconnected. The size of the macropores and the nanoporosity were higher in the scaffolds prepared with Klucel. Nanoporosity increased water uptake by the scaffolds, while macroporosity promoted cell ingrowth, which was most evident in scaffolds prepared with 25% Klucel. Human adipose-derived stem cells co-cultured with endothelial cells formed pre-vascular structures in the scaffolds, which was further enhanced in a dynamic cell culture system. The scaffolds are promising for the engineering of pre-vascularized soft tissues (relatively pliable 10% Klucel scaffolds) and hard tissues (mechanically stronger 25% and 50% Klucel scaffolds).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20505 - Composites (including laminates, reinforced plastics, cermets, combined natural and synthetic fibre fabrics; filled composites)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Sciences

ISSN

1661-6596

e-ISSN

1422-0067

Svazek periodika

26

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

41

Strana od-do

2974

Kód UT WoS článku

001463835600001

EID výsledku v databázi Scopus

2-s2.0-105002600359