Cultures of stored-product mite Tyrophagus putrescentiae experimentally infected with Cardinium and Wolbachia presented reduced fitness

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10178044" target="_blank" >RIV/00027006:_____/25:10178044 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/25:00143309

Výsledek na webu

<a href="https://www.sciencedirect.com/journal/journal-of-stored-products-research/vol/112/suppl/C" target="_blank" >https://www.sciencedirect.com/journal/journal-of-stored-products-research/vol/112/suppl/C</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jspr.2025.102663" target="_blank" >10.1016/j.jspr.2025.102663</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cultures of stored-product mite Tyrophagus putrescentiae experimentally infected with Cardinium and Wolbachia presented reduced fitness

Popis výsledku v původním jazyce

Tyrophagus putrescentiae is a cosmopolitan pest of stored food and animal feed. Mite populations differ in their microbiome composition, resulting in variability in their fitness. Cultures of the stored-product mite T. putrescentiae are often singly infected by one of intracellular bacterial genera Cardinium and Wolbachia. No naturally occurring multi-infected (Cardinium/Wolbachia) cultures have been observed. Under laboratory conditions, we mixed two singly infected mite cultures, i.e., Cardinium-infected (5L and 5S) and Wolbachia-infected (5N and 5P) cultures, to obtain four experimental cultures (5LN, 5LP, 5SN and 5SP), each infected with each of the endosymbionts. Population growth was used as a fitness indicator, and PCR with specific primers for a single mite was used to identify the prevalence of Cardinium and Wolbachia in the parental and mixed cultures. The population growth of the experimental cultures was lower than that of the parents at the beginning of the experiment. After six months, 5SN and 5SP exhibited greater fitness than 5LP and 5LN. The population growth of the 5SN and 5SP cultures did not differ from that of the parental cultures 5P and 5S. Cardinium-infected mites prevailed in 6 cultures, Wolbachia-infected mites in 5 cultures, and asymbiotic individuals in 1 culture after six months. The proportion of Cardinium-infected individuals (45 %) was greater than that of Wolbachia-infected individuals (25 %), indicating that Cardinium-infected mites had a fitness advantage over their Wolbachiainfected counterparts. Although the cultures were multi-infected, doubly infected mites were rare (7 %). These results suggest that Cardinium is responsible for cytoplasmic incompatibility in T. putrescentiae. The results revealed that the presence of the intracellular symbionts Cardinium and Wolbachia strongly influenced mite population growth in the experiments. These data support the importance of the microbiome in this pest.

Název v anglickém jazyce

Cultures of stored-product mite Tyrophagus putrescentiae experimentally infected with Cardinium and Wolbachia presented reduced fitness

Popis výsledku anglicky

Tyrophagus putrescentiae is a cosmopolitan pest of stored food and animal feed. Mite populations differ in their microbiome composition, resulting in variability in their fitness. Cultures of the stored-product mite T. putrescentiae are often singly infected by one of intracellular bacterial genera Cardinium and Wolbachia. No naturally occurring multi-infected (Cardinium/Wolbachia) cultures have been observed. Under laboratory conditions, we mixed two singly infected mite cultures, i.e., Cardinium-infected (5L and 5S) and Wolbachia-infected (5N and 5P) cultures, to obtain four experimental cultures (5LN, 5LP, 5SN and 5SP), each infected with each of the endosymbionts. Population growth was used as a fitness indicator, and PCR with specific primers for a single mite was used to identify the prevalence of Cardinium and Wolbachia in the parental and mixed cultures. The population growth of the experimental cultures was lower than that of the parents at the beginning of the experiment. After six months, 5SN and 5SP exhibited greater fitness than 5LP and 5LN. The population growth of the 5SN and 5SP cultures did not differ from that of the parental cultures 5P and 5S. Cardinium-infected mites prevailed in 6 cultures, Wolbachia-infected mites in 5 cultures, and asymbiotic individuals in 1 culture after six months. The proportion of Cardinium-infected individuals (45 %) was greater than that of Wolbachia-infected individuals (25 %), indicating that Cardinium-infected mites had a fitness advantage over their Wolbachiainfected counterparts. Although the cultures were multi-infected, doubly infected mites were rare (7 %). These results suggest that Cardinium is responsible for cytoplasmic incompatibility in T. putrescentiae. The results revealed that the presence of the intracellular symbionts Cardinium and Wolbachia strongly influenced mite population growth in the experiments. These data support the importance of the microbiome in this pest.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/GF22-15841K" target="_blank" >GF22-15841K: Roztoči jako modelová skupina pro sledování interakcí intracelulárních bakterií: Existuje válka mezi symbionty?</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF STORED PRODUCTS RESEARCH

ISSN

0022-474X

e-ISSN

1879-1212

Svazek periodika

112

Číslo periodika v rámci svazku

MAY 2025

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

102663

Kód UT WoS článku

001478892000001

EID výsledku v databázi Scopus

2-s2.0-105003095509