Soil cultivation methods rather than catch crop species enhance bacterial diversity in soil over a three-year experiment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10179170" target="_blank" >RIV/00027006:_____/25:10179170 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-025-15255-7.pdf" target="_blank" >https://www.nature.com/articles/s41598-025-15255-7.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-025-15255-7" target="_blank" >10.1038/s41598-025-15255-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Soil cultivation methods rather than catch crop species enhance bacterial diversity in soil over a three-year experiment

Popis výsledku v původním jazyce

Growing catch crops can improve soil health by enhancing microbial diversity, but their impact may be constrained by how they are managed. This study examined the effects of different catch crop species and soil cultivation methods on bacterial and fungal diversity, and total soil microbial biomass. A 3-year field experiment on Luvi-haplic Chernozem included two catch crop species and five mixtures (further catch crop species) and three cultivation methods (ploughing, reduced tillage and no tillage), resulting in 21 treatments (7 catch crop species x 3 soil cultivations). Soil samples (0-15 cm depth) were collected annually in autumn and spring since 2021 to 2024. Microbial biomass was assessed using the chloroform fumigation extraction method, while fungal and bacterial diversity was analysed by amplifying and sequencing the ITS2 region of rDNA and the V4 region of the 16 S rRNA gene for fungi and bacteria, respectively, via Illumina paired-end amplicon sequencing. Soil cultivation methods affected bacterial diversity, with reduced tillage showing higher diversity and evenness than ploughing, though neither differed from no-till. However, no effect was observed on fungal diversity, including AM fungi, or microbial biomass. Catch crop species did not significantly impact microbial diversity or biomass. Neither cultivation method nor catch crop species influenced the ratios of functional trophic groups, such as pathogens, saprotrophs or symbionts. This study underscores the critical role of soil management practices-especially reduced tillage-in promoting soil health, primarily through the enhancement of bacterial diversity.

Název v anglickém jazyce

Soil cultivation methods rather than catch crop species enhance bacterial diversity in soil over a three-year experiment

Popis výsledku anglicky

Growing catch crops can improve soil health by enhancing microbial diversity, but their impact may be constrained by how they are managed. This study examined the effects of different catch crop species and soil cultivation methods on bacterial and fungal diversity, and total soil microbial biomass. A 3-year field experiment on Luvi-haplic Chernozem included two catch crop species and five mixtures (further catch crop species) and three cultivation methods (ploughing, reduced tillage and no tillage), resulting in 21 treatments (7 catch crop species x 3 soil cultivations). Soil samples (0-15 cm depth) were collected annually in autumn and spring since 2021 to 2024. Microbial biomass was assessed using the chloroform fumigation extraction method, while fungal and bacterial diversity was analysed by amplifying and sequencing the ITS2 region of rDNA and the V4 region of the 16 S rRNA gene for fungi and bacteria, respectively, via Illumina paired-end amplicon sequencing. Soil cultivation methods affected bacterial diversity, with reduced tillage showing higher diversity and evenness than ploughing, though neither differed from no-till. However, no effect was observed on fungal diversity, including AM fungi, or microbial biomass. Catch crop species did not significantly impact microbial diversity or biomass. Neither cultivation method nor catch crop species influenced the ratios of functional trophic groups, such as pathogens, saprotrophs or symbionts. This study underscores the critical role of soil management practices-especially reduced tillage-in promoting soil health, primarily through the enhancement of bacterial diversity.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK21010308" target="_blank" >QK21010308: Efektivní systémy pěstování meziplodin využívající principy biotických intenzifikací</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

15

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

14

Strana od-do

30630

Kód UT WoS článku

001577164900034

EID výsledku v databázi Scopus

2-s2.0-105013866474