Efficient In Vitro Propagation and Cryopreservation of Garlic Using a Temporary Immersion System Bioreactor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10179569" target="_blank" >RIV/00027006:_____/25:10179569 - isvavai.cz</a>

Výsledek na webu

<a href="https://meetings.ipk-gatersleben.de/gatersleben-allium-symposium/program-2/" target="_blank" >https://meetings.ipk-gatersleben.de/gatersleben-allium-symposium/program-2/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Efficient In Vitro Propagation and Cryopreservation of Garlic Using a Temporary Immersion System Bioreactor

Popis výsledku v původním jazyce

The aim of this study was to grow garlic plants using a bioreactor quickly, which were then used for cryopreservation, using an optimized V-plate method first described by Tanaka (PCTOC,2021).A temporary immersion system (TIS) bioreactor has been used as an efficient and cost-effective method for the in vitro propagation of many plant species. In the current study, the applicability of a TIS bioreactor for the feasibility of in vitro propagation of garlic (Allium sativum L.) cultivars `Dukát` and `Ivan` was evaluated. The advantage of the temporary immersion bioreactor system (TIS) is that the plants are immersed in the liquid medium only for a given time period. The growth rate of plants increases in the TIS by improving the ventilation of the culture vessel. The TIS systems reduce some of the costs of in vitro propagation, thanks to not needing agar, and the time between replanting may be longer than in traditional in vitro cultivation methods.Apical shoot tips were excised from bioreactor-grown plants and precultured for 3 days at 22 °C on solidified MS medium containing 0.3 M sucrose. The shoot tips were then subjected to a loading solution (LS) (sucrose-glycerol) treatment for 20 minutes, followed by exposure to plant vitrification solution 3 (PVS3, 50/50 sucrose/glycerol) for 120 minutes at 25°C. The explants were then placed on modified aluminium foil with depression and plunged into liquid nitrogen for 1 hour. For recovery, shoot tips were immersed in 0.7 M sucrose solution for 20 minutes before being transferred to MS medium supplemented with NAA and BAP.The results demonstrated that garlic plants could be successfully propagated in the bioreactor within 7 days and subsequently used for cryopreservation. Using this protocol, the survival rate of cryopreserved shoot tips ranged from 80% to 100%, indicating the efficiency of the developed cryopreservation method.The proposed in vitro cultivation method has potential to be used as a pre-culture step for cryopreservation of garlic plants.

Název v anglickém jazyce

Efficient In Vitro Propagation and Cryopreservation of Garlic Using a Temporary Immersion System Bioreactor

Popis výsledku anglicky

The aim of this study was to grow garlic plants using a bioreactor quickly, which were then used for cryopreservation, using an optimized V-plate method first described by Tanaka (PCTOC,2021).A temporary immersion system (TIS) bioreactor has been used as an efficient and cost-effective method for the in vitro propagation of many plant species. In the current study, the applicability of a TIS bioreactor for the feasibility of in vitro propagation of garlic (Allium sativum L.) cultivars `Dukát` and `Ivan` was evaluated. The advantage of the temporary immersion bioreactor system (TIS) is that the plants are immersed in the liquid medium only for a given time period. The growth rate of plants increases in the TIS by improving the ventilation of the culture vessel. The TIS systems reduce some of the costs of in vitro propagation, thanks to not needing agar, and the time between replanting may be longer than in traditional in vitro cultivation methods.Apical shoot tips were excised from bioreactor-grown plants and precultured for 3 days at 22 °C on solidified MS medium containing 0.3 M sucrose. The shoot tips were then subjected to a loading solution (LS) (sucrose-glycerol) treatment for 20 minutes, followed by exposure to plant vitrification solution 3 (PVS3, 50/50 sucrose/glycerol) for 120 minutes at 25°C. The explants were then placed on modified aluminium foil with depression and plunged into liquid nitrogen for 1 hour. For recovery, shoot tips were immersed in 0.7 M sucrose solution for 20 minutes before being transferred to MS medium supplemented with NAA and BAP.The results demonstrated that garlic plants could be successfully propagated in the bioreactor within 7 days and subsequently used for cryopreservation. Using this protocol, the survival rate of cryopreserved shoot tips ranged from 80% to 100%, indicating the efficiency of the developed cryopreservation method.The proposed in vitro cultivation method has potential to be used as a pre-culture step for cryopreservation of garlic plants.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů