Assessment of the plasma membrane resistance of boar spermatozoa with using short hypoosmotic swelling test

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F09%3A%230001117" target="_blank" >RIV/00027014:_____/09:#0001117 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Assessment of the plasma membrane resistance of boar spermatozoa with using short hypoosmotic swelling test

Popis výsledku v původním jazyce

The objective of this study was to assess the sperm membrane resistance in diluted boar semen with using short hypoosmotic swelling test (sHOS-test). sHOS-test evaluates the functional integrity of the spermatozoa plasma membrane, especially of the tail.sHOS-test involves incubation of spermatozoa in hypoosmotic solution 75 mOsm/l for 5 min. Eosin-nigrosine staining test was used with sHOS-test. Ejaculates from 21 fertile hybrid AI boars were collected by the gloved hand method. In the fresh boar semenwere evaluated these parameters: semen volume, sperm motility, sperm concentration, morphologically abnormal spermatozoa, percentage of live sperm and sHOS-test. The boar semen was diluted in a semendilution rate of 1 + 4 in Androhep, Androstar, Androstar plus, MIII, LD and was stored at a temperature 17°C up to 96h. Samples of semen-dilution were kept at 38°C in water bath and resistance of spermatozoa was evaluated with sHOS-test before and after 1h incubation in water bath each day.

Název v anglickém jazyce

Assessment of the plasma membrane resistance of boar spermatozoa with using short hypoosmotic swelling test

Popis výsledku anglicky

The objective of this study was to assess the sperm membrane resistance in diluted boar semen with using short hypoosmotic swelling test (sHOS-test). sHOS-test evaluates the functional integrity of the spermatozoa plasma membrane, especially of the tail.sHOS-test involves incubation of spermatozoa in hypoosmotic solution 75 mOsm/l for 5 min. Eosin-nigrosine staining test was used with sHOS-test. Ejaculates from 21 fertile hybrid AI boars were collected by the gloved hand method. In the fresh boar semenwere evaluated these parameters: semen volume, sperm motility, sperm concentration, morphologically abnormal spermatozoa, percentage of live sperm and sHOS-test. The boar semen was diluted in a semendilution rate of 1 + 4 in Androhep, Androstar, Androstar plus, MIII, LD and was stored at a temperature 17°C up to 96h. Samples of semen-dilution were kept at 38°C in water bath and resistance of spermatozoa was evaluated with sHOS-test before and after 1h incubation in water bath each day.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GI - Šlechtění a plemenářství hospodářských zvířat

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Research in Pig Breeding

ISSN

1802-7547

e-ISSN

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Svazek periodika

3

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

3

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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