Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F12%3A%230001634" target="_blank" >RIV/00027014:_____/12:#0001634 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.vuzv.cz/sites/File/_privat/12075.pdf" target="_blank" >http://www.vuzv.cz/sites/File/_privat/12075.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.livsci.2011.12.018" target="_blank" >10.1016/j.livsci.2011.12.018</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis

Popis výsledku v původním jazyce

Genetic polymorphisms of bovine ?-casein (CSN3) and ?-lactoglobulin (LGB) have been shown to affect milk compositional traits and technological properties. Monitoring individual CSN3 and LGB genetic variants in the population is important in the subsequent process of breeding cattle and improving milk protein quality. The objective of this study was to develop and validate a method for genotyping the main mutations of the bovine CSN3 and LGB genes using real time PCR (RT-PCR) followed by a high-resolution melting method (HRM) assay. The HRM-based assay allowed the sensitive and fast identification of the CSN3 AA, AB, BB, AE and BE genotypes and LGB AA, AB and BB genotypes simultaneously. This method was validated by analyzing 100 Fleckvieh cattle DNA samples, which were also genotyped by conventional methods at the molecular level using PCR?RFLP and DNA sequencing techniques. The genotypes obtained using the HRM approach perfectly matched the genotypes obtained by conventional analyses

Název v anglickém jazyce

Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis

Popis výsledku anglicky

Genetic polymorphisms of bovine ?-casein (CSN3) and ?-lactoglobulin (LGB) have been shown to affect milk compositional traits and technological properties. Monitoring individual CSN3 and LGB genetic variants in the population is important in the subsequent process of breeding cattle and improving milk protein quality. The objective of this study was to develop and validate a method for genotyping the main mutations of the bovine CSN3 and LGB genes using real time PCR (RT-PCR) followed by a high-resolution melting method (HRM) assay. The HRM-based assay allowed the sensitive and fast identification of the CSN3 AA, AB, BB, AE and BE genotypes and LGB AA, AB and BB genotypes simultaneously. This method was validated by analyzing 100 Fleckvieh cattle DNA samples, which were also genotyped by conventional methods at the molecular level using PCR?RFLP and DNA sequencing techniques. The genotypes obtained using the HRM approach perfectly matched the genotypes obtained by conventional analyses

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Livestock Science

ISSN

1871-1413

e-ISSN

—

Svazek periodika

145

Číslo periodika v rámci svazku

1-3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

275-279

Kód UT WoS článku

000303270400037

EID výsledku v databázi Scopus

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