Factors affecting the number of STRO-1(+) stem cells derived from regenerating antler and pedicle cells of red and fallow deer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F12%3A%230001645" target="_blank" >RIV/00027014:_____/12:#0001645 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.vuzv.cz/sites/File/_privat/12087.pdf" target="_blank" >http://www.vuzv.cz/sites/File/_privat/12087.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1071/AN12012" target="_blank" >10.1071/AN12012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Factors affecting the number of STRO-1(+) stem cells derived from regenerating antler and pedicle cells of red and fallow deer

Popis výsledku v původním jazyce

Mesenchymal stem cells positive to surface antigen STRO-1 were isolated from regenerating antlers of red deer (Cervus elaphus) and fallow deer (Dama dama) using a magnetic-activated cell sorting (MACS) method. In this study we analysed factors potentially affecting the number of STRO-1(+) cells in the cell cultures. With regard to the STRO-1 antigen, we evaluated data from 188 MACS separation procedures of cell cultures cultivated in Dulbecco's Modified Eagle Medium and 10% fetal calf serum of four fallow deer males (130 procedures) and four red deer males (58 procedures). The analysed factors were the sampling site of the antler or the pedicle, cell passage and type of the cell culture (mixed or STRO-1 negative cell cultures). The percentage of obtained STRO-1(+) cells varied greatly from 0.4 to 38.9% for fallow deer and from 1.8 to 16.5% for red deer. We have not found any significant influence of the sampling site. The passage and the type of culture were significant factors for bot

Název v anglickém jazyce

Factors affecting the number of STRO-1(+) stem cells derived from regenerating antler and pedicle cells of red and fallow deer

Popis výsledku anglicky

Mesenchymal stem cells positive to surface antigen STRO-1 were isolated from regenerating antlers of red deer (Cervus elaphus) and fallow deer (Dama dama) using a magnetic-activated cell sorting (MACS) method. In this study we analysed factors potentially affecting the number of STRO-1(+) cells in the cell cultures. With regard to the STRO-1 antigen, we evaluated data from 188 MACS separation procedures of cell cultures cultivated in Dulbecco's Modified Eagle Medium and 10% fetal calf serum of four fallow deer males (130 procedures) and four red deer males (58 procedures). The analysed factors were the sampling site of the antler or the pedicle, cell passage and type of the cell culture (mixed or STRO-1 negative cell cultures). The percentage of obtained STRO-1(+) cells varied greatly from 0.4 to 38.9% for fallow deer and from 1.8 to 16.5% for red deer. We have not found any significant influence of the sampling site. The passage and the type of culture were significant factors for bot

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA523%2F08%2F0808" target="_blank" >GA523/08/0808: Hormonální podstata vztahu mezi sociální aktivitou a růstem paroží III</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animal Production Science 

ISSN

1836-0939

e-ISSN

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Svazek periodika

52

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

AU - Austrálie

Počet stran výsledku

4

Strana od-do

746-749

Kód UT WoS článku

000306282400012

EID výsledku v databázi Scopus

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