Role of Choline and Methionine in Bovine Mammary Epithelial Cell Line Exposed to Hydrogen Peroxide
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F13%3A%230001941" target="_blank" >RIV/00027014:_____/13:#0001941 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.vuzv.cz/sites/File/_privat/13176.pdf" target="_blank" >http://www.vuzv.cz/sites/File/_privat/13176.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1166/jnef.2013.1033" target="_blank" >10.1166/jnef.2013.1033</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Role of Choline and Methionine in Bovine Mammary Epithelial Cell Line Exposed to Hydrogen Peroxide
Popis výsledku v původním jazyce
Choline and methionine are essential nutrients for many mammals. In this work we aimed to evaluate 1^ role of choline and methionine in counteracting the oxidative stress induced by hydrogen perowein bovine mammary epithelial cells. BME-UV1 cell line hasbeen previously used as in vitro model for mammary epithelium. First of all, BME-UV1 were exposed to increasing concentrations of hydrogen peroxide to establish half lethal concentrations at 24, 48 and 72 hours. Furthermore, BME-UV1 cells were incubatedwith increasing amounts of choline and methionine in order to identify the most effective concentrations in term of cell viability. In this respect, two combinations of choline and methionine have been selected: a Low Choline/Methionine dosage and a High Choline/Methionine dosage, providing respectively to 500 yu.M/715 ytiM and 1000 ytiM/1430 ytiM of choline and methionine. After that, BME-UV1 treated with Low Choline/Methionine dosage and High Choline/Methionine dosage were exposed to
Název v anglickém jazyce
Role of Choline and Methionine in Bovine Mammary Epithelial Cell Line Exposed to Hydrogen Peroxide
Popis výsledku anglicky
Choline and methionine are essential nutrients for many mammals. In this work we aimed to evaluate 1^ role of choline and methionine in counteracting the oxidative stress induced by hydrogen perowein bovine mammary epithelial cells. BME-UV1 cell line hasbeen previously used as in vitro model for mammary epithelium. First of all, BME-UV1 were exposed to increasing concentrations of hydrogen peroxide to establish half lethal concentrations at 24, 48 and 72 hours. Furthermore, BME-UV1 cells were incubatedwith increasing amounts of choline and methionine in order to identify the most effective concentrations in term of cell viability. In this respect, two combinations of choline and methionine have been selected: a Low Choline/Methionine dosage and a High Choline/Methionine dosage, providing respectively to 500 yu.M/715 ytiM and 1000 ytiM/1430 ytiM of choline and methionine. After that, BME-UV1 treated with Low Choline/Methionine dosage and High Choline/Methionine dosage were exposed to
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
—
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Nutritional Ecology and Food Research
ISSN
2326-4225
e-ISSN
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Svazek periodika
1
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
5
Strana od-do
189-193
Kód UT WoS článku
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EID výsledku v databázi Scopus
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