The effect of various substances on inhibition of microorganisms and sperm survival in short-term boar semen extender

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F19%3AN0000291" target="_blank" >RIV/00027014:_____/19:N0000291 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.respigbreed.cz/2019/1/1.pdf" target="_blank" >http://www.respigbreed.cz/2019/1/1.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of various substances on inhibition of microorganisms and sperm survival in short-term boar semen extender

Popis výsledku v původním jazyce

The objective of this study was to investigate the effect of sodium thiosulfate (TS1, 2, 3), colloidal zinc + ascorbic acid (CZ1, 2) and honey (H) added to boar semen extender BTS without antibiotic (BTS0) as a substitute for antibiotics on inhibition of microorganisms and their influence on sperm survival. The amount tested substances added to boar semen extender was not effect on initial sperm motility (p <0.05) unlike higher amount of substances. Nine ejaculates from 4 healthy and fertile AI boars were used for this study. Tested substances were added to BTS0 extender in dilution rate of 1+2, 1+4 and 1+8 and stored at 17°C up to 48h, for everyday evaluation. Sperm survival was affected by storage time, dilution ratio and type of substances (p <0.05). Significant differences (p <0.05) of total mean values of sperm motility were found between samples BTS0 70.21% vs. BTS0+TS3 61.39% and BTS0+H 49.09%. The sample with the BTS0+H had statistically lower sperm motility 49.09% compared to other tested samples (p <0.05). Microorganisms were not significantly inhibited by any of tested substances added to the extender. In conclusion, utilization of tested substances as a potential substitute for antibiotics in boar semen extender is not possible. Therefore, it is necessary to research other potential substances for a possible replacement for antibiotic in boar semen extender.

Název v anglickém jazyce

The effect of various substances on inhibition of microorganisms and sperm survival in short-term boar semen extender

Popis výsledku anglicky

The objective of this study was to investigate the effect of sodium thiosulfate (TS1, 2, 3), colloidal zinc + ascorbic acid (CZ1, 2) and honey (H) added to boar semen extender BTS without antibiotic (BTS0) as a substitute for antibiotics on inhibition of microorganisms and their influence on sperm survival. The amount tested substances added to boar semen extender was not effect on initial sperm motility (p <0.05) unlike higher amount of substances. Nine ejaculates from 4 healthy and fertile AI boars were used for this study. Tested substances were added to BTS0 extender in dilution rate of 1+2, 1+4 and 1+8 and stored at 17°C up to 48h, for everyday evaluation. Sperm survival was affected by storage time, dilution ratio and type of substances (p <0.05). Significant differences (p <0.05) of total mean values of sperm motility were found between samples BTS0 70.21% vs. BTS0+TS3 61.39% and BTS0+H 49.09%. The sample with the BTS0+H had statistically lower sperm motility 49.09% compared to other tested samples (p <0.05). Microorganisms were not significantly inhibited by any of tested substances added to the extender. In conclusion, utilization of tested substances as a potential substitute for antibiotics in boar semen extender is not possible. Therefore, it is necessary to research other potential substances for a possible replacement for antibiotic in boar semen extender.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Research in Pig Breeding

ISSN

1802-7547

e-ISSN

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Svazek periodika

13

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

1-6

Kód UT WoS článku

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EID výsledku v databázi Scopus

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