Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F20%3AN0000072" target="_blank" >RIV/00027014:_____/20:N0000072 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/20:81072 RIV/00216208:11140/20:10403491

Výsledek na webu

<a href="http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=5&SID=D3vFDVS6MmhCaxyr9tU&page=1&doc=17" target="_blank" >http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=5&SID=D3vFDVS6MmhCaxyr9tU&page=1&doc=17</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/cells9010237" target="_blank" >10.3390/cells9010237</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes

Popis výsledku v původním jazyce

The role of hydrogen sulfide (H2S) is addressed in Xenopuslaevis oocytes. Three enzymes involvedinH2Smetabolism,cystathionineβ-synthase,cystathionineγ-lyase,and3-mercaptopyruvate sulfurtransferase, were detected in prophase I and metaphase II-arrested oocytes and drove an accelerationofoocytemeiosisresumptionwheninhibited. Moreover,meiosisresumptionisassociated with a significant decrease in endogenous H2S. On another hand, a dose-dependent inhibition was obtained using the H2S donor, NaHS (1 and 5 mM). NaHS impaired translation. NaHS did not inducethedissociationofthecomponentsoftheM-phasepromotingfactor(MPF),cyclinBandCdk1, nor directly impacted the MPF activity. However, the M-phase entry induced by microinjection of metaphase II MPF-containing cytoplasm was diminished, suggesting upstream components of the MPF auto-amplification loop were sensitive to H2S. Superoxide dismutase and catalase hindered the effects of NaHS, and this sensitivity was partially dependent on the production of reactive oxygen species (ROS). In contrast to other species, no apoptosis was promoted. These results suggest a contribution of H2S signaling in the timing of amphibian oocytes meiosis resumption.

Název v anglickém jazyce

Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes

Popis výsledku anglicky

The role of hydrogen sulfide (H2S) is addressed in Xenopuslaevis oocytes. Three enzymes involvedinH2Smetabolism,cystathionineβ-synthase,cystathionineγ-lyase,and3-mercaptopyruvate sulfurtransferase, were detected in prophase I and metaphase II-arrested oocytes and drove an accelerationofoocytemeiosisresumptionwheninhibited. Moreover,meiosisresumptionisassociated with a significant decrease in endogenous H2S. On another hand, a dose-dependent inhibition was obtained using the H2S donor, NaHS (1 and 5 mM). NaHS impaired translation. NaHS did not inducethedissociationofthecomponentsoftheM-phasepromotingfactor(MPF),cyclinBandCdk1, nor directly impacted the MPF activity. However, the M-phase entry induced by microinjection of metaphase II MPF-containing cytoplasm was diminished, suggesting upstream components of the MPF auto-amplification loop were sensitive to H2S. Superoxide dismutase and catalase hindered the effects of NaHS, and this sensitivity was partially dependent on the production of reactive oxygen species (ROS). In contrast to other species, no apoptosis was promoted. These results suggest a contribution of H2S signaling in the timing of amphibian oocytes meiosis resumption.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

<a href="/cs/project/LO1503" target="_blank" >LO1503: BIOMEDIC</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cells

ISSN

2073-4409

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

22

Strana od-do

Article Number 237

Kód UT WoS článku

000515398200237

EID výsledku v databázi Scopus

2-s2.0-85098467490