Quantification of cannabinoids and cholesterol in biological materials of different origins via reversed-phase high-performance liquid chromatography with photodiode array detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F25%3A10006221" target="_blank" >RIV/00027014:_____/25:10006221 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S0889157525001309" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0889157525001309</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jfca.2025.107316" target="_blank" >10.1016/j.jfca.2025.107316</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Quantification of cannabinoids and cholesterol in biological materials of different origins via reversed-phase high-performance liquid chromatography with photodiode array detection

Popis výsledku v původním jazyce

The purpose of our study was to develop original precolumn procedures and C18 high-performance liquid chromatography with a photodiode array detection method for the simultaneous determination of sixteen physiologically important cannabinoids and free cholesterol in plant and animal samples. The precolumn procedure for cannabinoid analysis in nonanimal samples involved two methanol and two n-hexane extractions due to the stronger extraction properties of the methanol solvent. The precolumn procedure in samples of animal origin (homogenized animal tissues, egg yolk of hens and feces), where cholesterol was analyzed together with cannabinoids, consisted of four n-hexane extractions due to the solubility of cannabinoids and cholesterol in n-hexane solvent. Methanol and n-hexane proved to be the appropriate solvents for the extraction of cannabinoids and cholesterol, which occur in assayed biological samples in wide concentration ranges. All the samples were chromatographically analyzed via gradient elution with acetonitrile with formic acid, water with formic acid and methanol. Our chromatographic method, irrespective of the precolumn procedure used, allowed for the selective, precise, accurate and sensitive quantification of cannabinoids and cholesterol in standard solutions and plant and animal samples. The sensitive and selective quantification of cannabinoids is important, especially in food products, because accurate monitoring of psychotropic cannabinoids is particularly necessary.

Název v anglickém jazyce

Quantification of cannabinoids and cholesterol in biological materials of different origins via reversed-phase high-performance liquid chromatography with photodiode array detection

Popis výsledku anglicky

The purpose of our study was to develop original precolumn procedures and C18 high-performance liquid chromatography with a photodiode array detection method for the simultaneous determination of sixteen physiologically important cannabinoids and free cholesterol in plant and animal samples. The precolumn procedure for cannabinoid analysis in nonanimal samples involved two methanol and two n-hexane extractions due to the stronger extraction properties of the methanol solvent. The precolumn procedure in samples of animal origin (homogenized animal tissues, egg yolk of hens and feces), where cholesterol was analyzed together with cannabinoids, consisted of four n-hexane extractions due to the solubility of cannabinoids and cholesterol in n-hexane solvent. Methanol and n-hexane proved to be the appropriate solvents for the extraction of cannabinoids and cholesterol, which occur in assayed biological samples in wide concentration ranges. All the samples were chromatographically analyzed via gradient elution with acetonitrile with formic acid, water with formic acid and methanol. Our chromatographic method, irrespective of the precolumn procedure used, allowed for the selective, precise, accurate and sensitive quantification of cannabinoids and cholesterol in standard solutions and plant and animal samples. The sensitive and selective quantification of cannabinoids is important, especially in food products, because accurate monitoring of psychotropic cannabinoids is particularly necessary.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food Composition and Analysis

ISSN

0889-1575

e-ISSN

—

Svazek periodika

140

Číslo periodika v rámci svazku

April

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1-10

Kód UT WoS článku

001419607200001

EID výsledku v databázi Scopus

2-s2.0-85216544372