Development and characterization of microsatellite markers in black poplar (Populus nigraL.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027073%3A_____%2F00%3A00000052" target="_blank" >RIV/00027073:_____/00:00000052 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development and characterization of microsatellite markers in black poplar (Populus nigraL.)

Popis výsledku v původním jazyce

Using an enrichment procedure, we have cloned and sequenced microsatellite loci from black poplar (Populus nigra L.) and developed primers for sequence-tagged microsatellite (STMS) analysis. Twelve primer pairs for dinucleotide repeats produced fragmentsof sufficient quality which were polymorphic in P. nigra.Some of them also showed amplification in other poplar species (P. deltoides, P. tricocarpa, P. tremula, P. tremuloides, P. candicans, and/or P. lasiocarpa). The best nine (GT) (GA) microsatellitemarkers were tested on a set of 23 P. nigra genotypes from all over Europe.The microsatellites were highly polymorphic, with 10 to 19 different alleles per microsatellite locus among these 23 genotypes. Using the other eight marker loci, the level of heterozygosity among the plants was on average 0.71 (range 0.25 to 1.00). The microsatellite markers developed will be useful for screening the genetic diversity in natural populations and gene bank collect

Název v anglickém jazyce

Development and characterization of microsatellite markers in black poplar (Populus nigraL.)

Popis výsledku anglicky

Using an enrichment procedure, we have cloned and sequenced microsatellite loci from black poplar (Populus nigra L.) and developed primers for sequence-tagged microsatellite (STMS) analysis. Twelve primer pairs for dinucleotide repeats produced fragmentsof sufficient quality which were polymorphic in P. nigra.Some of them also showed amplification in other poplar species (P. deltoides, P. tricocarpa, P. tremula, P. tremuloides, P. candicans, and/or P. lasiocarpa). The best nine (GT) (GA) microsatellitemarkers were tested on a set of 23 P. nigra genotypes from all over Europe.The microsatellites were highly polymorphic, with 10 to 19 different alleles per microsatellite locus among these 23 genotypes. Using the other eight marker loci, the level of heterozygosity among the plants was on average 0.71 (range 0.25 to 1.00). The microsatellite markers developed will be useful for screening the genetic diversity in natural populations and gene bank collect

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Theoretical and applied genetics. : International journal of breeding research and cell genetics.

ISSN

0040-5752

e-ISSN

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Svazek periodika

101

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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