In vitro root formation in Pulsatilla vernalis (L.) MILL.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027073%3A_____%2F12%3A%230001458" target="_blank" >RIV/00027073:_____/12:#0001458 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.journal-pop.org/2012_12_2_96-101.html" target="_blank" >http://www.journal-pop.org/2012_12_2_96-101.html</a>

DOI - Digital Object Identifier

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Jazyk výsledku

čeština

Název v původním jazyce

In vitro root formation in Pulsatilla vernalis (L.) MILL.

Popis výsledku v původním jazyce

An efficient in vitro rooting system was developed for the endangered and difficult-to-root spring pasque flower (Pulsatilla vernalis (L.) Mill). Rhizogenesis of shoots was observed on half-strength woody plant agar medium only with high concentration ofindole-3-butyric acid (IBA). Optimal rooting was observed for incised shoots grown on half-strength woody plant perlite medium with 20 mg l-1 IBA for 3 weeks and then on an expressive medium. Under these conditions 84.7% of the shoots rooted. The incision of shoots (on the base of shoot) had significantly beneficial effects on root induction. Application of auxin during in vitro rhizogenesis affected subsequent acclimatization of plantlets. The highest plantlet survival rate (90.5%) was observed in plants which were dipped in IBA and then exposured to the induction medium for 3 weeks during in vitro phase. The plants were replanted and transferred to a shady hotbed, and were ready for planting in the next year.

Název v anglickém jazyce

In vitro root formation in Pulsatilla vernalis (L.) MILL.

Popis výsledku anglicky

An efficient in vitro rooting system was developed for the endangered and difficult-to-root spring pasque flower (Pulsatilla vernalis (L.) Mill). Rhizogenesis of shoots was observed on half-strength woody plant agar medium only with high concentration ofindole-3-butyric acid (IBA). Optimal rooting was observed for incised shoots grown on half-strength woody plant perlite medium with 20 mg l-1 IBA for 3 weeks and then on an expressive medium. Under these conditions 84.7% of the shoots rooted. The incision of shoots (on the base of shoot) had significantly beneficial effects on root induction. Application of auxin during in vitro rhizogenesis affected subsequent acclimatization of plantlets. The highest plantlet survival rate (90.5%) was observed in plants which were dipped in IBA and then exposured to the induction medium for 3 weeks during in vitro phase. The plants were replanted and transferred to a shady hotbed, and were ready for planting in the next year.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Propagation of Ornamental Plants

ISSN

1311-9109

e-ISSN

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Svazek periodika

12

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

BG - Bulharská republika

Počet stran výsledku

6

Strana od-do

96-101

Kód UT WoS článku

000306541700003

EID výsledku v databázi Scopus

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