Induced polyploidization in Moringa oleifera using oryzalin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027073%3A_____%2F24%3AN0000037" target="_blank" >RIV/00027073:_____/24:N0000037 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.actahort.org/books/1394/1394_6.htm" target="_blank" >https://www.actahort.org/books/1394/1394_6.htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2024.1394.6" target="_blank" >10.17660/ActaHortic.2024.1394.6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Induced polyploidization in Moringa oleifera using oryzalin

Popis výsledku v původním jazyce

Moringa oleifera Lam. (drumstick tree) is a fast-growing deciduous tree of the family Moringaceae, native to the Indian subcontinent. Moringa trees are grown mainly in semiarid, tropical, and subtropical areas. It has multiple uses due to its rich content of bioactive compounds and nutrients and its adaptability to a wide range of agroclimatic conditions. Moringa is referred to as “a miracle tree”; therefore, it is desirable to obtain new genotypes with original properties. The aim of this study was to obtain tetraploids using induced in vitro polyploidization by treatment with an antimitotic agent. In vitro shoots were shaken in liquid full-strength Murashige and Skoog (MS) induction medium with oryzalin added at a concentration 50 µM and 100 µM for 24 h or 96 h of cultivation. The ploidy level was confirmed by flow cytometry. Then, tetraploids were compared with diploids in terms of basic morphological and physiological characteristics. It was found that the oryzalin concentration and exposure time have no effect on the induction of tetraploid plants. On the other hand, both factors influenced the regeneration of the in vitro culture. Tetraploids were obtained in all treatments, but the efficiency was low. The ploidy level had a significant effect on plant growth and morphology.

Název v anglickém jazyce

Induced polyploidization in Moringa oleifera using oryzalin

Popis výsledku anglicky

Moringa oleifera Lam. (drumstick tree) is a fast-growing deciduous tree of the family Moringaceae, native to the Indian subcontinent. Moringa trees are grown mainly in semiarid, tropical, and subtropical areas. It has multiple uses due to its rich content of bioactive compounds and nutrients and its adaptability to a wide range of agroclimatic conditions. Moringa is referred to as “a miracle tree”; therefore, it is desirable to obtain new genotypes with original properties. The aim of this study was to obtain tetraploids using induced in vitro polyploidization by treatment with an antimitotic agent. In vitro shoots were shaken in liquid full-strength Murashige and Skoog (MS) induction medium with oryzalin added at a concentration 50 µM and 100 µM for 24 h or 96 h of cultivation. The ploidy level was confirmed by flow cytometry. Then, tetraploids were compared with diploids in terms of basic morphological and physiological characteristics. It was found that the oryzalin concentration and exposure time have no effect on the induction of tetraploid plants. On the other hand, both factors influenced the regeneration of the in vitro culture. Tetraploids were obtained in all treatments, but the efficiency was low. The ploidy level had a significant effect on plant growth and morphology.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

978-94-62613-93-5

ISSN

0567-7572

e-ISSN

2406-6168

Počet stran výsledku

8

Strana od-do

43-50

Název nakladatele

International Society for Horticultural Science

Místo vydání

Leuven

Místo konání akce

Aracaju

Datum konání akce

8. 11. 2023

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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