Somatic embryogenesis and histological analysis in Rhododendron L. and Dendranthema L.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027073%3A_____%2F99%3A077" target="_blank" >RIV/00027073:_____/99:077 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Somatic embryogenesis and histological analysis in Rhododendron L. and Dendranthema L.

Popis výsledku v původním jazyce

Influence of growth regulators on somatic embryo initiation and histological analysis were determined in Rhododendron catawbiense Michx. 'Grandiflorum' and Dendranthema grandiflorum 'Reagan'Somatic embryos in rhododendron were initiated in the presence of cytokinins TDZ (thidiazuron) 5 mg.l-1 and adeninsulphate 40 mg.l-1 after 6 weeks of culture. In cv. Reagan somatic embryos were induced after transfer from medium supplemented by 2,4-D (2,4-dichlorophenoxyacetic acid) 1 mg.l-1 and BAP (6-benzylaminopurine) 0.2 mg.l-1 on medium without hormones. Culture full-strenght MS medium (Murashige and Skoog, 1962) was used in both cultivars. Tissues for histological studies were vacuum-infiltrated with FAA, dehydrated in a graded series of ethanol and embedded in paraffin wax. The embryogenic mass and somatic embryos were mostly derived from several morphologically competent cells

Název v anglickém jazyce

Somatic embryogenesis and histological analysis in Rhododendron L. and Dendranthema L.

Popis výsledku anglicky

Influence of growth regulators on somatic embryo initiation and histological analysis were determined in Rhododendron catawbiense Michx. 'Grandiflorum' and Dendranthema grandiflorum 'Reagan'Somatic embryos in rhododendron were initiated in the presence of cytokinins TDZ (thidiazuron) 5 mg.l-1 and adeninsulphate 40 mg.l-1 after 6 weeks of culture. In cv. Reagan somatic embryos were induced after transfer from medium supplemented by 2,4-D (2,4-dichlorophenoxyacetic acid) 1 mg.l-1 and BAP (6-benzylaminopurine) 0.2 mg.l-1 on medium without hormones. Culture full-strenght MS medium (Murashige and Skoog, 1962) was used in both cultivars. Tissues for histological studies were vacuum-infiltrated with FAA, dehydrated in a graded series of ethanol and embedded in paraffin wax. The embryogenic mass and somatic embryos were mostly derived from several morphologically competent cells

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

1999

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biológia

ISSN

006-3088

e-ISSN

—

Svazek periodika

54

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

1

Strana od-do

—

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—