Aneuploidy detection in porcine embryos using fluorescence in situ hybridization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F01%3A80000040" target="_blank" >RIV/00027162:_____/01:80000040 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Aneuploidy detection in porcine embryos using fluorescence in situ hybridization

Popis výsledku v původním jazyce

Very few data on the incidence and risk factors for aneuploidy, as a significant cause of reproductive disorders in farm animals, are available. Most aneuploid conceptuses die in utero and methods for early assessnebt of aneuploidy are needed. Porcine embryos were collected, transferred onto poly-L lysine-coated slides and fixed with 0.01 N HCl, 0.1 % Tween 20. A method of multi-color fluorescence in situ hybridisation was developed for detecting chromosomes 1, 10 and Y in blastomeres. Only embryos withextra signals were regarded as pathological. Eighty-five of the 93 examined embryos (91.4%) were diploid, 2 (2.6%) were tripolid, 5 (5.4%) were mosaic, 1 (1.1%) showed trisomy, and 44 (47.3%) showed 1 chromosome Y signal.

Název v anglickém jazyce

Aneuploidy detection in porcine embryos using fluorescence in situ hybridization

Popis výsledku anglicky

Very few data on the incidence and risk factors for aneuploidy, as a significant cause of reproductive disorders in farm animals, are available. Most aneuploid conceptuses die in utero and methods for early assessnebt of aneuploidy are needed. Porcine embryos were collected, transferred onto poly-L lysine-coated slides and fixed with 0.01 N HCl, 0.1 % Tween 20. A method of multi-color fluorescence in situ hybridisation was developed for detecting chromosomes 1, 10 and Y in blastomeres. Only embryos withextra signals were regarded as pathological. Eighty-five of the 93 examined embryos (91.4%) were diploid, 2 (2.6%) were tripolid, 5 (5.4%) were mosaic, 1 (1.1%) showed trisomy, and 44 (47.3%) showed 1 chromosome Y signal.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chromosome research (14th International Chromosome Conference)

ISSN

0967-3849

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

suppl.1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

2

Strana od-do

77-78

Kód UT WoS článku

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EID výsledku v databázi Scopus

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