Studium izolátů komplexu Mycobacterium avium od skotu z České republiky pocházejících z let 1996 až 2000

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F04%3A00010915" target="_blank" >RIV/00027162:_____/04:00010915 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Study of Mycobacterium avium complex strains isolated from cattle in the Czech Republic between 1996 to 2000

Popis výsledku v původním jazyce

This study has surveyed 2 593 348 slaughtered animals during the years 1996 to 2000, and found 571 animals with tuberculous lesions. Culture of 346 selected tissue samples from animals younger and older than two years isolated mycobacteria from 91 animals M. avium subsp. avium, M. a hominissuis, M. a. paratuberculosis, M. chelonae, and M. terrae isolates. In both age categories culture of mesenteric lymph node samples showed 66 MAC isolates. Mycobacteria were more frequently isolated from tissues of animals in the age under two years (34.4%). By the IS901 and IS1245 RFLP methods, 17 randomly selected MAC isolates from 17 different cattle herds were examined; they produced 11 distinct IS901 RFLP types and three IS1245 RFLP profiles. All 17 isolates werevirulent after intramuscular inoculation of pullets. By cloning of 17 isolates, IS901 / IS1245 RFLP profiles identical with those of the original isolates were obtained in 15 of them. M. a. paratuberculosis isolated was found to be RFLP

Název v anglickém jazyce

Study of Mycobacterium avium complex strains isolated from cattle in the Czech Republic between 1996 to 2000

Popis výsledku anglicky

This study has surveyed 2 593 348 slaughtered animals during the years 1996 to 2000, and found 571 animals with tuberculous lesions. Culture of 346 selected tissue samples from animals younger and older than two years isolated mycobacteria from 91 animals M. avium subsp. avium, M. a hominissuis, M. a. paratuberculosis, M. chelonae, and M. terrae isolates. In both age categories culture of mesenteric lymph node samples showed 66 MAC isolates. Mycobacteria were more frequently isolated from tissues of animals in the age under two years (34.4%). By the IS901 and IS1245 RFLP methods, 17 randomly selected MAC isolates from 17 different cattle herds were examined; they produced 11 distinct IS901 RFLP types and three IS1245 RFLP profiles. All 17 isolates werevirulent after intramuscular inoculation of pullets. By cloning of 17 isolates, IS901 / IS1245 RFLP profiles identical with those of the original isolates were obtained in 15 of them. M. a. paratuberculosis isolated was found to be RFLP

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FN - Epidemiologie, infekční nemoci a klinická imunologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Microbiology

ISSN

0378-1135

e-ISSN

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Svazek periodika

99

Číslo periodika v rámci svazku

3-4

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

12

Strana od-do

239-250

Kód UT WoS článku

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EID výsledku v databázi Scopus

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