PCR metoda v reálném čase pro kvantifikaci DNA prasečího cirkoviru 2 v klinických vzorcích z prasat

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Real time PCR assay for quantification of porcine circovirus type 2 DNA in pig clinical samples using the light cycler

Popis výsledku v původním jazyce

The objective of our study was to test the newly developed "real time" PCR method for quantification of PCV2/DNA templates in the organs of diseased and clinically normal pigs, and moreover, to confirm a possible share of PCV2 infections in the occurrence of pleropneumonia in older pigs in the Czech Republic. The detection limit of the method is 50 DNA copies/ul of the cloned part of ORF2/PCV2 in pDNAK8L plasmid (external standard). During the clinical course of PMWS, PCV2 genome was detected using a real time PCR in 100% cases, predominantly in lymphoid tissues at high levels reaching in 1 g of the tissue under testing from 2,060E+06 to 2,146E+12 genome copy number. Markedly high doses of PCV2 (4,500E+10 copies) were detected in lung tissues in 43% ofpleuropneumonia cases in pre-fattening and fattening pigs, often in combination with Actinobacillus pleropneumonie, especially of serotypes 9 and 11, or Pasteurella multocida A or D and/or Hemophilus parasuis.

Název v anglickém jazyce

Real time PCR assay for quantification of porcine circovirus type 2 DNA in pig clinical samples using the light cycler

Popis výsledku anglicky

The objective of our study was to test the newly developed "real time" PCR method for quantification of PCV2/DNA templates in the organs of diseased and clinically normal pigs, and moreover, to confirm a possible share of PCV2 infections in the occurrence of pleropneumonia in older pigs in the Czech Republic. The detection limit of the method is 50 DNA copies/ul of the cloned part of ORF2/PCV2 in pDNAK8L plasmid (external standard). During the clinical course of PMWS, PCV2 genome was detected using a real time PCR in 100% cases, predominantly in lymphoid tissues at high levels reaching in 1 g of the tissue under testing from 2,060E+06 to 2,146E+12 genome copy number. Markedly high doses of PCV2 (4,500E+10 copies) were detected in lung tissues in 43% ofpleuropneumonia cases in pre-fattening and fattening pigs, often in combination with Actinobacillus pleropneumonie, especially of serotypes 9 and 11, or Pasteurella multocida A or D and/or Hemophilus parasuis.

Druh

D - Stať ve sborníku

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

18th International Pig Veterinary Society Congress, June 27 - July 1, 2004, Hamburg

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

49-49

Název nakladatele

IPVS Veranstaltungs GmbH

Místo vydání

Hamburg, Germany

Místo konání akce

Hamburg, Germany

Datum konání akce

27. 6. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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