Charakteristika izolátů Mycobacterium caprae získaných v Evropě pomocí metody MIRU (mycobacterial interspersed repetitive unit) genotyping

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F05%3A06000045" target="_blank" >RIV/00027162:_____/05:06000045 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of Mycobacterium caprae by mycobacterial interspersed repetitive units (MIRU) for epidemiological and phylogenetic purposes

Popis výsledku v původním jazyce

M.caprae causes tuberculosis among animals and humans in several European countries. To characterize M.caprae we analyzed 232 M caprae isolates by MIRU and by spoligotyping. The isolates originated from 128 epidemiological settings in 10 countries. Wefound 78 different MIRU types but only 17 spoligotypes, giving Hunter-Gaston discriminatory indices of 0.941 (MIRU) and 0.665 (spoligotyping). For a subset of 103 M.caprae isolates derived from outbreaks, MIRU and IS6110 RFLP were compared and shown toprovide similar results. MIRU loci 4, 26, and 31 were most discriminant in M.caprae, followed by loci 10 and 16, a combination which is different than those reported to discriminate M.bovis best. M.caprae MIRU patterns together with published data wereused for the neighbor-joining method. M.caprae isolates were grouped together, closely related to the branches of classical members MTC, but apart from modern M.tuberculosis. Our data confirm M.caprae as a distinct phylogenetic lineage within th

Název v anglickém jazyce

Characterization of Mycobacterium caprae by mycobacterial interspersed repetitive units (MIRU) for epidemiological and phylogenetic purposes

Popis výsledku anglicky

M.caprae causes tuberculosis among animals and humans in several European countries. To characterize M.caprae we analyzed 232 M caprae isolates by MIRU and by spoligotyping. The isolates originated from 128 epidemiological settings in 10 countries. Wefound 78 different MIRU types but only 17 spoligotypes, giving Hunter-Gaston discriminatory indices of 0.941 (MIRU) and 0.665 (spoligotyping). For a subset of 103 M.caprae isolates derived from outbreaks, MIRU and IS6110 RFLP were compared and shown toprovide similar results. MIRU loci 4, 26, and 31 were most discriminant in M.caprae, followed by loci 10 and 16, a combination which is different than those reported to discriminate M.bovis best. M.caprae MIRU patterns together with published data wereused for the neighbor-joining method. M.caprae isolates were grouped together, closely related to the branches of classical members MTC, but apart from modern M.tuberculosis. Our data confirm M.caprae as a distinct phylogenetic lineage within th

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FN - Epidemiologie, infekční nemoci a klinická imunologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Clinical Microbiology

ISSN

0095-1137

e-ISSN

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Svazek periodika

43

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

4984-4992

Kód UT WoS článku

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EID výsledku v databázi Scopus

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