Role apoptózy neutrofilních granulocytů během experimentální infekce mléčné žlázy Streptococcus uberis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F06%3A%230000065" target="_blank" >RIV/00027162:_____/06:#0000065 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The role of neutrophil apoptosis during experimentally induced Strptococcus uberis Mastitis

Popis výsledku v původním jazyce

The objective of this study was to determine whether neutrophil apoptosis and their consequent elimination by macrophages from the mammary gland is modulated by an infection caused by Staphylococcus aureus (S. aureus). The study was performed on twenty mammary glands of 5 virgin heifers. A buffered physiological solution (PBS) was administered as a means of control into the mammary glands of the heifers and after 168 hours the glands were inoculated with S. aureus. Flow cytometry was used for determination of Annexin-V positivity and propidium iodide (PI) negativity of neutrophils. Light microscopy was used for determination of neutrophil karyopyknosis. Histochemistry was used for detection of myeloperoxidase positive (MPO+) macrophages. The dynamics of resolution of mastitis caused by S. aureus was very similar to resolution of acute damage of the mammary gland after PBS administration. The modulatory effect of infection on the course of this infection was very weak.

Název v anglickém jazyce

The role of neutrophil apoptosis during experimentally induced Strptococcus uberis Mastitis

Popis výsledku anglicky

The objective of this study was to determine whether neutrophil apoptosis and their consequent elimination by macrophages from the mammary gland is modulated by an infection caused by Staphylococcus aureus (S. aureus). The study was performed on twenty mammary glands of 5 virgin heifers. A buffered physiological solution (PBS) was administered as a means of control into the mammary glands of the heifers and after 168 hours the glands were inoculated with S. aureus. Flow cytometry was used for determination of Annexin-V positivity and propidium iodide (PI) negativity of neutrophils. Light microscopy was used for determination of neutrophil karyopyknosis. Histochemistry was used for detection of myeloperoxidase positive (MPO+) macrophages. The dynamics of resolution of mastitis caused by S. aureus was very similar to resolution of acute damage of the mammary gland after PBS administration. The modulatory effect of infection on the course of this infection was very weak.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/GA524%2F01%2F1290" target="_blank" >GA524/01/1290: Vliv antimikrobiálních látek na indukci apoptózy a nekrózy polymorfonukleárních leukocytů v rezoluci zánětu mléčné žlázy skotu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární medicína

ISSN

0375-8427

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

9

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

11

Strana od-do

9

Kód UT WoS článku

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EID výsledku v databázi Scopus

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