Detekce viru jarní virémie kaprů (SVCV) pomocí monoklonálních protilátek

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F07%3A%230000162" target="_blank" >RIV/00027162:_____/07:#0000162 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of spring viremia of carp virus (SVCV) with monoclonal antibodies

Popis výsledku v původním jazyce

Monoclonal antibodies (MAbs) against spring viraemia of carp virus (SVCV)were prepared. Nine MAbs were characterised using Western blotting (WB), all reacted with glycoprotein G, except for MAb 2E1, which showed no reactivity in WB. All nine MAbs showedspecificity in an immunoperoxidase test. In ELISA assays, their titres ranged between 1:32 000 and 1:128 000. A panel of SVCV isolates from different European regions were set up and examined by sandwich ELISA assay using the MAbs. Only MAb 4C12/3C8 showed low sensitivity in most of the isolates at an absorbance of A450. No cross-reaction with the infectious haematopoietic necrosis virus (IHNV), viral haemorrhagic septicaemia virus (VHSV) or infectious pancreatic necrosis virus (IPNV) was demonstrated.2E1 did not show cross-reactivity with PFRV classified in genogroup III - IV and reacted with a Czech SVCV isolate. Other Abs reacted positively with PFRV F4 reference strain, isolated from Esox lucius L (genogrup III).

Název v anglickém jazyce

Detection of spring viremia of carp virus (SVCV) with monoclonal antibodies

Popis výsledku anglicky

Monoclonal antibodies (MAbs) against spring viraemia of carp virus (SVCV)were prepared. Nine MAbs were characterised using Western blotting (WB), all reacted with glycoprotein G, except for MAb 2E1, which showed no reactivity in WB. All nine MAbs showedspecificity in an immunoperoxidase test. In ELISA assays, their titres ranged between 1:32 000 and 1:128 000. A panel of SVCV isolates from different European regions were set up and examined by sandwich ELISA assay using the MAbs. Only MAb 4C12/3C8 showed low sensitivity in most of the isolates at an absorbance of A450. No cross-reaction with the infectious haematopoietic necrosis virus (IHNV), viral haemorrhagic septicaemia virus (VHSV) or infectious pancreatic necrosis virus (IPNV) was demonstrated.2E1 did not show cross-reactivity with PFRV classified in genogroup III - IV and reacted with a Czech SVCV isolate. Other Abs reacted positively with PFRV F4 reference strain, isolated from Esox lucius L (genogrup III).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární medicína

ISSN

0375-8427

e-ISSN

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Svazek periodika

52

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

308-316

Kód UT WoS článku

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EID výsledku v databázi Scopus

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