TNF-alfa moduluje efekty ligandů receptoru AhR na buněčnou proliferaci a expresi enzymů cytochromů P450 v potkaních jaterních 'stem-like' buňkách

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F07%3A%230000283" target="_blank" >RIV/00027162:_____/07:#0000283 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Tumor necrosis factor-alpha modulates effects of aryl hydrocarbon receptor ligands on cell proliferation and expression of cytochrome P450 enzymes in rat liver 'stem-like' cells

Popis výsledku v původním jazyce

Various liver diseases lead to an extensive inflammatory response and release of pro-inflammatory cytokines, such as TNF-alpha. We investigated possible interactions of TNF-alpha with ligands of the AhR receptor and known liver carcinogens, such as 2,3,7,8-TCDD and co-planar PCB 126. TNF-alpha significantly potentiated proliferative effects of picomolar range doses of both TCDD and PCB 126, leading to an increase in cell numbers, as well as an increased percentage of cells entering the S-phase of the cell cycle. The combination of TNF-alpha with low concentrations of AhR ligands increased both mRNA and protein levels of cyclin A. TNF-alpha temporarily inhibited AhR-dependent induction of CYP1A1. In contrast, TNF-alpha significantly enhanced induction of CYP1B1. These results suggest that TNF-alpha can significantly amplify effects of AhR ligands on deregulation of cell proliferation control, as well as on expression of CYP1B1, involved in metabolic activation of a number of mutagenic c

Název v anglickém jazyce

Tumor necrosis factor-alpha modulates effects of aryl hydrocarbon receptor ligands on cell proliferation and expression of cytochrome P450 enzymes in rat liver 'stem-like' cells

Popis výsledku anglicky

Various liver diseases lead to an extensive inflammatory response and release of pro-inflammatory cytokines, such as TNF-alpha. We investigated possible interactions of TNF-alpha with ligands of the AhR receptor and known liver carcinogens, such as 2,3,7,8-TCDD and co-planar PCB 126. TNF-alpha significantly potentiated proliferative effects of picomolar range doses of both TCDD and PCB 126, leading to an increase in cell numbers, as well as an increased percentage of cells entering the S-phase of the cell cycle. The combination of TNF-alpha with low concentrations of AhR ligands increased both mRNA and protein levels of cyclin A. TNF-alpha temporarily inhibited AhR-dependent induction of CYP1A1. In contrast, TNF-alpha significantly enhanced induction of CYP1B1. These results suggest that TNF-alpha can significantly amplify effects of AhR ligands on deregulation of cell proliferation control, as well as on expression of CYP1B1, involved in metabolic activation of a number of mutagenic c

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicological Sciences

ISSN

1096-6080

e-ISSN

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Svazek periodika

99

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

79-89

Kód UT WoS článku

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EID výsledku v databázi Scopus

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