Srovnání pozitivity Mycobacterium avium subsp. paratuberculosis u klinicky zdravých ovcí a koz z jihozápadního Řecka s využitím molekulárních technik, sérologie a kultivace

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F07%3A%230000287" target="_blank" >RIV/00027162:_____/07:#0000287 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparative evaluation of positive tests to Mycobacterium avium subsp. paratuberculosis in clinically healthy sheep and goats in south-west Greece using molecular techniques, serology, and culture

Popis výsledku v původním jazyce

Mycobacterium a. paratuberculosis (MAP) is the cause of paratuberculosis that affects mainly ruminants. Recently it was shown that retail cheese consist a considerable means of human exposure to MAP. Therefore the aim of this study was to assess positivity to MAP of clinically healthy sheep and goats using comparatively the techniques. From a total of 30 flocks, 632 sheep and goats had faecal, serum, and whole-blood samples examined, respectively, by culture, the complement fixation test (CFT), and PCRtargeted to IS900, IS1245, and IS6110. PCR produced positive results in 21% of the animals that were tested, with 5.6%, 3.9%, and 11.5%, being identified as MAP, M.a. avium, and M.tuberculosis complex. CFT produced positive and suspicious results in 4.4%and 14.4% of the cases. Faecal cultures were negative in all but a single case that was identified as RFLP-type BC1. The agreement between the results obtained by PCR and CFT was poor with reference to isolated cases.

Název v anglickém jazyce

Comparative evaluation of positive tests to Mycobacterium avium subsp. paratuberculosis in clinically healthy sheep and goats in south-west Greece using molecular techniques, serology, and culture

Popis výsledku anglicky

Mycobacterium a. paratuberculosis (MAP) is the cause of paratuberculosis that affects mainly ruminants. Recently it was shown that retail cheese consist a considerable means of human exposure to MAP. Therefore the aim of this study was to assess positivity to MAP of clinically healthy sheep and goats using comparatively the techniques. From a total of 30 flocks, 632 sheep and goats had faecal, serum, and whole-blood samples examined, respectively, by culture, the complement fixation test (CFT), and PCRtargeted to IS900, IS1245, and IS6110. PCR produced positive results in 21% of the animals that were tested, with 5.6%, 3.9%, and 11.5%, being identified as MAP, M.a. avium, and M.tuberculosis complex. CFT produced positive and suspicious results in 4.4%and 14.4% of the cases. Faecal cultures were negative in all but a single case that was identified as RFLP-type BC1. The agreement between the results obtained by PCR and CFT was poor with reference to isolated cases.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Journal

ISSN

1090-0233

e-ISSN

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Svazek periodika

174

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

337-343

Kód UT WoS článku

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EID výsledku v databázi Scopus

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