Detekce a genetická charakteristika viru hepatitidy E v českých komerčních chovech prasat

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F08%3A%230000373" target="_blank" >RIV/00027162:_____/08:#0000373 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection and genetic characterisation of Hepatitis E virus in Czech pig production herds

Popis výsledku v původním jazyce

The objective of the present study was to carry out a small surveillance programme in Czech pig production herds using the nested RT-PCR to trace hepatitis E virus (HEV) in different biological samples. Due to the high genetic variability of HEV, three sets of primers of its genome were used. A total of 32 piglets from 11 herds clinically suspected of postweaning multisystemic wasting syndrome (PMWS) were examined. Bile, liver and serum samples were collected from each animal. HEV RNA was most frequently detected in the bile samples (40.0%), followed by liver (16.1%) and serum (3.2%). Seven (63.6%) of the 11 monitored farms were found to have at least one HEV RNA positive piglet. Specific 242 bp sequences within the ORF1 were sequenced and phylogenetically analysed. Phylogenetic analysis confirmed that all detected Czech swine HEV (CZswHEV) isolates belonged to genotype III. Comparison of the CZswHEV isolates with sequences of swHEV available in GenBank failed to find any 100% homologo

Název v anglickém jazyce

Detection and genetic characterisation of Hepatitis E virus in Czech pig production herds

Popis výsledku anglicky

The objective of the present study was to carry out a small surveillance programme in Czech pig production herds using the nested RT-PCR to trace hepatitis E virus (HEV) in different biological samples. Due to the high genetic variability of HEV, three sets of primers of its genome were used. A total of 32 piglets from 11 herds clinically suspected of postweaning multisystemic wasting syndrome (PMWS) were examined. Bile, liver and serum samples were collected from each animal. HEV RNA was most frequently detected in the bile samples (40.0%), followed by liver (16.1%) and serum (3.2%). Seven (63.6%) of the 11 monitored farms were found to have at least one HEV RNA positive piglet. Specific 242 bp sequences within the ORF1 were sequenced and phylogenetically analysed. Phylogenetic analysis confirmed that all detected Czech swine HEV (CZswHEV) isolates belonged to genotype III. Comparison of the CZswHEV isolates with sequences of swHEV available in GenBank failed to find any 100% homologo

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/1B53016" target="_blank" >1B53016: Výzkum a inovace nových molekulárně diagnostických metod k studiu cirkovirů, parvoviru a PRRSV v polymikrobiální etiologii infekčních chorob prasat a pro kontrolu účinnosti preventivních a léčebných opatření.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Research in Veterinary Science

ISSN

0034-5288

e-ISSN

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Svazek periodika

87

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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