Characterizace konjugovaných plazmidlů přenášejících rezistenci k antibiotikům a jejich interakce s plazmidem virulence u Salmonella enterica serovar Typhimurium

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F08%3A%230000408" target="_blank" >RIV/00027162:_____/08:#0000408 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Characterisation of Salmonella enterica serovar Typhimurium conjugative plasmids transferring resistance to antibiotics and their interaction with the virulence plasmid

Popis výsledku v původním jazyce

In this study we analysed field isolates of Salmonella enterica serovar Typhimurium (S. Typhimurium) for the presence of conjugative plasmids transferring resistances to antibiotics. Altogether 23 strains were analysed for the presence of conjugative R plasmids. Conjugation and transfer of antibiotic resistance was observed in 12 strains. In two cases, resistance to tetracycline and/or ampicillin was not transferred by conjugation to ~10% of transconjugants. Detailed characterisation showed that the loss of both resistances was associated with the loss of Tn3 (blaTEM) and Tn1721 (tet(A)). However when only the tetracycline resistance was lost, the Tn1721 was replaced with a partial sequence of rck, and coding sequences of srgA, srgB, ORF7 and pefI originating from the S. Typhimurium virulence plasmid. Two plasmids from our collection were capable of recombination with the virulence plasmid of S. Typhimurium and subsequently spread both antibiotic resistance and virulence genes to the r

Název v anglickém jazyce

Characterisation of Salmonella enterica serovar Typhimurium conjugative plasmids transferring resistance to antibiotics and their interaction with the virulence plasmid

Popis výsledku anglicky

In this study we analysed field isolates of Salmonella enterica serovar Typhimurium (S. Typhimurium) for the presence of conjugative plasmids transferring resistances to antibiotics. Altogether 23 strains were analysed for the presence of conjugative R plasmids. Conjugation and transfer of antibiotic resistance was observed in 12 strains. In two cases, resistance to tetracycline and/or ampicillin was not transferred by conjugation to ~10% of transconjugants. Detailed characterisation showed that the loss of both resistances was associated with the loss of Tn3 (blaTEM) and Tn1721 (tet(A)). However when only the tetracycline resistance was lost, the Tn1721 was replaced with a partial sequence of rck, and coding sequences of srgA, srgB, ORF7 and pefI originating from the S. Typhimurium virulence plasmid. Two plasmids from our collection were capable of recombination with the virulence plasmid of S. Typhimurium and subsequently spread both antibiotic resistance and virulence genes to the r

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/1B44019" target="_blank" >1B44019: Rezistence salmonel k antimikrobiálním látkám</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Antimicrobial Chemotherapy

ISSN

0305-7453

e-ISSN

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Svazek periodika

62

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

4

Strana od-do

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Kód UT WoS článku

000259974700015

EID výsledku v databázi Scopus

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