Genotyping of Mycobacterium avium subsp avium isolates from domestic animals in Slovenia by IS901 RFLP

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F09%3A%230000483" target="_blank" >RIV/00027162:_____/09:#0000483 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genotyping of Mycobacterium avium subsp avium isolates from domestic animals in Slovenia by IS901 RFLP

Popis výsledku v původním jazyce

The detection of IS901 restriction fragment length polymorphism (RFLP) types of Mycobacterium avium subsp. avium (MAA) isolates from different domestic animals between 1998 and 2004 was the aim of this study. Detected RFLP types were compared with previously described RFLP types (the database of the OIE Reference Laboratory for Avian Tuberculosis, Brno, Czech Republic). A total of 62 isolates (56 from pigs, 5 from poultry and one from cattle) were identified with IS901 PCR and IS901 RFLP typed using PvuII and PstI endonucleases. Seven PvuII and 11 PstI RFLP types resulted in 12 combined PvuII PstI types; none of these matched the combined RFLP types described in previous studies. Two new PvuII and eight new PstI RFLP types were described. Identical RFLP types were found among isolates originating from individual farms. A unique RFLP type F-A17 was detected in isolates from different pig herds and also in poultry isolates. Detection of identical RFLP types on different farms may reflect

Název v anglickém jazyce

Genotyping of Mycobacterium avium subsp avium isolates from domestic animals in Slovenia by IS901 RFLP

Popis výsledku anglicky

The detection of IS901 restriction fragment length polymorphism (RFLP) types of Mycobacterium avium subsp. avium (MAA) isolates from different domestic animals between 1998 and 2004 was the aim of this study. Detected RFLP types were compared with previously described RFLP types (the database of the OIE Reference Laboratory for Avian Tuberculosis, Brno, Czech Republic). A total of 62 isolates (56 from pigs, 5 from poultry and one from cattle) were identified with IS901 PCR and IS901 RFLP typed using PvuII and PstI endonucleases. Seven PvuII and 11 PstI RFLP types resulted in 12 combined PvuII PstI types; none of these matched the combined RFLP types described in previous studies. Two new PvuII and eight new PstI RFLP types were described. Identical RFLP types were found among isolates originating from individual farms. A unique RFLP type F-A17 was detected in isolates from different pig herds and also in poultry isolates. Detection of identical RFLP types on different farms may reflect

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární medicína

ISSN

0375-8427

e-ISSN

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Svazek periodika

54

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

000268784300002

EID výsledku v databázi Scopus

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