Effect of muramyl dipeptide on apoptosis of bovine mammary gland lymphocytes in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F09%3A%230000558" target="_blank" >RIV/00027162:_____/09:#0000558 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of muramyl dipeptide on apoptosis of bovine mammary gland lymphocytes in vitro

Popis výsledku v původním jazyce

The aim of this study was to determine whether apoptosis of lymphocytes is modulated by muramyl dipeptide (MDP) that is a minimal structural unit of peptidoglycan in Gram-positive bacteria. To this aim, apoptosis of lymphocytes was studied in in vitro environment (RPMI medium, 37 °C, 5% CO2) with 10 ug.ml-1 MDP. Heifers were used as mammary gland cell donors for in vitro studies. Lymphocytes from the intact mammary glands were harvested following the phosphate buffered saline intramammary injection. Apoptosis and necrosis of lymphocytes were analysed by flow cytometry following simultaneous staining with Annexin-V and propidium iodide. The results of this study demonstrate that apoptosis of lymphocytes was delayed during in vitro incubation with MDP.

Název v anglickém jazyce

Effect of muramyl dipeptide on apoptosis of bovine mammary gland lymphocytes in vitro

Popis výsledku anglicky

The aim of this study was to determine whether apoptosis of lymphocytes is modulated by muramyl dipeptide (MDP) that is a minimal structural unit of peptidoglycan in Gram-positive bacteria. To this aim, apoptosis of lymphocytes was studied in in vitro environment (RPMI medium, 37 °C, 5% CO2) with 10 ug.ml-1 MDP. Heifers were used as mammary gland cell donors for in vitro studies. Lymphocytes from the intact mammary glands were harvested following the phosphate buffered saline intramammary injection. Apoptosis and necrosis of lymphocytes were analysed by flow cytometry following simultaneous staining with Annexin-V and propidium iodide. The results of this study demonstrate that apoptosis of lymphocytes was delayed during in vitro incubation with MDP.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Slovak Journal of Animal Science

ISSN

1337-9984

e-ISSN

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Svazek periodika

42

Číslo periodika v rámci svazku

Suppl.1

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

4

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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