Occurrence of Mycobacterium avium subspecies paratuberculosis across host species and European countries with evidence for transmission between wildlife and domestic ruminants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F09%3A%230000582" target="_blank" >RIV/00027162:_____/09:#0000582 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Occurrence of Mycobacterium avium subspecies paratuberculosis across host species and European countries with evidence for transmission between wildlife and domestic ruminants

Popis výsledku v původním jazyce

This study was undertaken to determine the genetic diversity of MAP, enhance understanding of the host range and distribution and assess the potential for interspecies transmission. 164 MAP isolates from 7 European countries representing 19 different host species were genotyped by standardized IS900 - restriction fragment length polymorphism (IS900-RFLP), pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphisms (AFLP) and mycobacterial interspersed repeat unit-variable number tandem repeat (MIRU-VNTR) analyses. Six PstI and 17 BstEII IS900-RFLP, 31 multiplex [SnaBI-SpeI] PFGE profiles and 23 MIRU-VNTR profiles were detected. AFLP gave insufficient discrimination of isolates. Two predominant strain types were detected across Europe. Interspecies transmission between wildlife and domestic ruminants on the same property was demonstrated in 4 cases, between wildlife species on the same property in two cases and between different species of domestic livestock on one

Název v anglickém jazyce

Occurrence of Mycobacterium avium subspecies paratuberculosis across host species and European countries with evidence for transmission between wildlife and domestic ruminants

Popis výsledku anglicky

This study was undertaken to determine the genetic diversity of MAP, enhance understanding of the host range and distribution and assess the potential for interspecies transmission. 164 MAP isolates from 7 European countries representing 19 different host species were genotyped by standardized IS900 - restriction fragment length polymorphism (IS900-RFLP), pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphisms (AFLP) and mycobacterial interspersed repeat unit-variable number tandem repeat (MIRU-VNTR) analyses. Six PstI and 17 BstEII IS900-RFLP, 31 multiplex [SnaBI-SpeI] PFGE profiles and 23 MIRU-VNTR profiles were detected. AFLP gave insufficient discrimination of isolates. Two predominant strain types were detected across Europe. Interspecies transmission between wildlife and domestic ruminants on the same property was demonstrated in 4 cases, between wildlife species on the same property in two cases and between different species of domestic livestock on one

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BMC Microbiology

ISSN

1471-2180

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

212

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

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Kód UT WoS článku

000271288300001

EID výsledku v databázi Scopus

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