Distribution of Mycobacterium avium subsp avium and M. a. hominissuis in artificially infected pigs studied by culture and 1S901 and IS1245 quantitative real time PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F10%3A%230000638" target="_blank" >RIV/00027162:_____/10:#0000638 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Distribution of Mycobacterium avium subsp avium and M. a. hominissuis in artificially infected pigs studied by culture and 1S901 and IS1245 quantitative real time PCR

Popis výsledku v původním jazyce

Mycobacterium avium subsp. avium (MAA) and M. a. hominissuis (MAH) are frequently associated with diseases in animals and humans. The aim of this study was to develop a system for rapid and accurate real time quantitative PCR (qPCR) identification and quantification of MAA and MAH. This study included 22 per os infected pigs (ten were infected with MAA, ten with MAH and two negative controls). From each animal, 21 different tissue samples and blood were tested by microscopy, culture and qPCR. The developed triplex qPCR reaction was based on the simultaneous detection of specific insertion sequences, IS901 and IS1245, and also contained an internal amplification control. Contrary to culture examination, triplex qPCR confirmed the infection in all animals infected with MAA, and in eight animals infected with MAH. From the food safety point of view the presence of MAH in muscles should be considered as a possible threat to human health.

Název v anglickém jazyce

Distribution of Mycobacterium avium subsp avium and M. a. hominissuis in artificially infected pigs studied by culture and 1S901 and IS1245 quantitative real time PCR

Popis výsledku anglicky

Mycobacterium avium subsp. avium (MAA) and M. a. hominissuis (MAH) are frequently associated with diseases in animals and humans. The aim of this study was to develop a system for rapid and accurate real time quantitative PCR (qPCR) identification and quantification of MAA and MAH. This study included 22 per os infected pigs (ten were infected with MAA, ten with MAH and two negative controls). From each animal, 21 different tissue samples and blood were tested by microscopy, culture and qPCR. The developed triplex qPCR reaction was based on the simultaneous detection of specific insertion sequences, IS901 and IS1245, and also contained an internal amplification control. Contrary to culture examination, triplex qPCR confirmed the infection in all animals infected with MAA, and in eight animals infected with MAH. From the food safety point of view the presence of MAH in muscles should be considered as a possible threat to human health.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Microbiology

ISSN

0378-1135

e-ISSN

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Svazek periodika

144

Číslo periodika v rámci svazku

3-4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000281358600026

EID výsledku v databázi Scopus

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