Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000731" target="_blank" >RIV/00027162:_____/11:#0000731 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces

Popis výsledku v původním jazyce

Analysis of 476 faecal samples from diarrhoeic piglets was performed using electron microscopy (EM) and a competitive blocking (CB)-ELISA based on monoclonal antibodies to the VP6 protein of group A rotavirus. Rotavirus was detected by EM and/or CB-ELISAin 111 (23.3%) samples. Of these, all groups of rotavirus were identified in 83 (74.8%) samples by EM (EM plus), while group A rotavirus was identified in 90 (81.1%) samples by CB-ELISA (ELISA plus). However, only 62 (55.9%) of samples were positive using both detection methods. The finding of 28 (25.2%) EM-/CB-ELISA plus samples illustrated the high sensitivity of the CB-ELISA method. On PCR analysis, groups B and C rotavirus was found in 3 and 16 0119 EMplus/CB-ELISA- samples, respectively. Althoughthe study illustrates the high sensitivity of a CB-ELISA in rotavirus detection, the findings highlight the need to use a range of diagnostic methods in detecting these viruses in clinical samples. (C) 2009 Elsevier Ltd. All rights reserv

Název v anglickém jazyce

Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces

Popis výsledku anglicky

Analysis of 476 faecal samples from diarrhoeic piglets was performed using electron microscopy (EM) and a competitive blocking (CB)-ELISA based on monoclonal antibodies to the VP6 protein of group A rotavirus. Rotavirus was detected by EM and/or CB-ELISAin 111 (23.3%) samples. Of these, all groups of rotavirus were identified in 83 (74.8%) samples by EM (EM plus), while group A rotavirus was identified in 90 (81.1%) samples by CB-ELISA (ELISA plus). However, only 62 (55.9%) of samples were positive using both detection methods. The finding of 28 (25.2%) EM-/CB-ELISA plus samples illustrated the high sensitivity of the CB-ELISA method. On PCR analysis, groups B and C rotavirus was found in 3 and 16 0119 EMplus/CB-ELISA- samples, respectively. Althoughthe study illustrates the high sensitivity of a CB-ELISA in rotavirus detection, the findings highlight the need to use a range of diagnostic methods in detecting these viruses in clinical samples. (C) 2009 Elsevier Ltd. All rights reserv

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/QF4051" target="_blank" >QF4051: Využití biotechnologických postupů v diagnostice a prevenci virových gastroenteritid prasat</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Journal

ISSN

1090-0233

e-ISSN

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Svazek periodika

187

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

3

Strana od-do

279-281

Kód UT WoS článku

000288520000029

EID výsledku v databázi Scopus

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